端粒酶反义寡聚脱氧核苷酸对人肝癌细胞株端粒酶活性作用的实验研究  

The effect of telomerase antisense oligodeoxynucleotides on the growth of hepatoma cell line

作　　者：许兰涛[1] 

机构地区：[1]兰州医学院第二附属医院,甘肃兰州730030

出　　处：《临床肝胆病杂志》2001年第2期107-109,共3页Journal of Clinical Hepatology

摘　　要：观察端粒酶反义寡聚脱氧核苷酸对肝癌细胞株的作用。用端粒酶反义寡聚脱氧核苷酸与肝癌细胞株共同孵育一定时间后 ,观察细胞形态变化、细胞DNA含量的分布 ,测定端粒酶活性。端粒酶反义寡聚脱氧核苷酸能诱导肝癌细胞凋亡 ,抑制端粒酶活性 ,在形态学上表现为细胞膜起泡、染色质固缩、核碎裂、凋亡小体形成 ;电泳呈凋亡特征性Ladder带 ;流式细胞仪分析显示 ,在G1期前出现亚 2倍体凋亡峰 ;端粒酶活性抑制。端粒酶反义寡聚脱氧核苷酸能诱导肝癌细胞凋亡 ,抑制端粒酶活性 ,抑制端粒合成 ,从而抑制肝癌细胞的生长。To observe the effect of telomerase antisense oligodeoxynucleotides on hepatoma cell line. Hepatoma cell line were cultured with (1～5) μmol·L -1 telomerase antisense oligodeoxynucleotides for various duration, cell activity, morphologic changes and cell-DNA distribution were studied. The apoptotic cells were measured with flow cytometer. Telomerase activity were detected by TRAP-ELISA. Telomerase antisense oligodeoxynucleotides acted as a specific growth inhibitor to hepatoma cell line. Hepatoma cells cultured with 5μmol·L -1 telomerase antisense oligodexynucleotides presented apoptotic features:i.e Intace cell membrane, chromatin condensation, nucleic fragmentation and apoptotic body formation; flow cytometry analysis showed a sub-G 1 cell peak; agarose clectrophoresis showed marked DNA ladder. Telomerase activity were inhibited. Telomerase may play a crucial role in the growth of hepatoma cells. Telomerase antisense oligodeoxynucleotides may inhibit telomerase activity and which may be a new target of treatment to hepatoma.

关 键 词：端粒酶 活性 反义寡聚脱氧核苷酸 细胞凋亡肝癌细胞 

分 类 号：R735.7[医药卫生—肿瘤]