维甲酸诱导白血病细胞分化对WT1基因表达的影响  

THE EFFECT OF ATRA-INDUCED DIFFERENTIATION OF LEUKEMIA CELLS ON WT1 EXPRESSION IN LEUKEMIA

作　　者：吴晓雄[1] 汪月增[1] 裴雪涛[2] 

机构地区：[1]解放军总医院血液科 [2]军事医学科学院放射医学研究所

出　　处：《解放军医学杂志》2001年第5期355-357,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：为了解WT1基因的表达与白血病细胞分化的关系以及WT1基因在白血病细胞分化中的作用 ,本文采用全反式维甲酸 (ATRA)诱导HL 6 0、K5 6 2细胞系分化 ,然后应用NBT还原试验和RT PCR方法分别测定细胞分化程度和WT1基因表达的变化。结果发现 ,ATRA作用 5天后可以诱导HL 6 0细胞分化 ,WT1的表达随着HL 6 0细胞的分化而降低 ;ATRA对K5 6 2细胞无诱导分化作用 ,其WT1的表达也无显著变化。提示WT1基因的表达与造血细胞分化呈负相关 ,与白血病细胞的分化有密切联系。To elucidate the relationship between WT1 expression and differentiation of leukemia cells and the role of WT1 gene in differentiation of leukemia cells, HL 60 and K562 cell lines were induced for 5 days by all trans retinoic acid(ATRA).Then the degree of differentiation and WT1 expression of cell lines were determined by NBT reduction assay and RT PCR respectively. The resultsshowed that only differentiation of HL 60 cells but no K562 cells could be induced by ATRA. When HL 60 cells were induced to differentiate to granulocytes by ATRA, the expression of WT1 decreased markedly during differentiation. However, WT1 transcripts were not significantly altered in K562 cells in which differentiation wasn't found. It suggested that WT1 gene expression may relate to the differentiation of leukemia cells.

关 键 词：白血病 WT1基因 细胞分化 基因表达 维甲酸 

分 类 号：R733.7[医药卫生—肿瘤]