戴氏虫草和粉被虫草多糖对巨噬细胞等活性的影响  

Enhancement of Cordyceps taii polysaccharide and Cordyceps pruinosa polysaccharide on cellular immune function in vitro

作　　者：刘杰麟[1] 费樱[2] 

机构地区：[1]贵阳医学院临床微生物免疫室,贵州贵阳550004 [2]贵阳医学院附属医院免疫科,贵州贵阳550004

出　　处：《免疫学杂志》2001年第3期189-191,共3页Immunological Journal

基　　金：国家自然科学基金! (3936 0 0 80 );贵州省自然科学基金! [2 9(1992 ) :30 0 2 ]资助项目

摘　　要：目的研究虫草属新种 -戴氏虫草菌丝体胞外水溶性多糖 (CDP1)和粉被虫草菌丝体多糖 (CPP1)在体外对小鼠腹腔巨噬细胞 (MΦ)吞噬功能和脾细胞免疫功能的影响。方法 MTT比色法测定淋巴细胞增殖 ,孔雀绿比色法测定小鼠腹腔巨噬细胞吞噬功能 ,中性红比色法测定细胞毒淋巴细胞 (CTL)活性。结果 CDP1在正常情况下不能促进小鼠腹腔巨噬细胞的吞噬功能 ,而 CPP1却有促进作用 ;在免疫抑制的情况下 ,一定浓度的戴氏虫草菌丝体胞外水溶性多糖和粉被虫草多糖能恢复提高吞噬功能。它们不仅能促进正常脾活化 T淋巴细胞的增殖 ,而且能恢复环磷酰胺和氢化可的松抑制免疫小鼠脾活化 T淋巴细胞的增殖。在合适的浓度下能增高小鼠脾 CTL 活性 ,同时在一定浓度下能恢复免疫抑制小鼠脾 CTL 活性。结论两者具有增强免疫功能的作用。Objective Cordyceps polysaccharide has important effects on inhibition of tumor, radio-protection and enhancement of immune response. In this paper ,the effect of Cordyceps taii polysaccharide (CDP1) and Cordyceps pruinosa polysaccharide(CPP1) on cellular immunological function of splenic T cell and MΦ phagocytosis in mice was investigated. MethodsThe proliferation of lymphocytes was measured with MTT assay, the activity of CTL cells was detected by colorimetric method of neutral red, MΦ phagocytosis was measured with colorimetric method of malachite green.ResultsNot only was the proliferation of activated splenic T cell enhanced, but also proliferation of activated splenic T cell in immune-inhibited mice was restored with CDP1 and CPP1 in vitro . It could promote CTL cytotoxity and M Φ phagocytosis in normal mice and immune inhibited mice.Conclusion Cordyceps taii polysaccharide and Cordyceps pruinosa polysaccharide can enhance the cellular immune functions in vitro . [

关 键 词：戴氏虫草多糖 粉被虫草多糖 增强免疫 巨噬细胞 细胞毒淋巴细胞 吞噬功能 小鼠 

分 类 号：R285.5[医药卫生—中药学]