嗜热厌氧纤维素降解菌对纤维素的粘附及其酶活性研究  

Adherence of thermophilic cellulolytic anaerobe to cellulose and cellulase activity

作　　者：韩如旸[1] 梅建凤[1] 闵航[1] 陈美慈[1] 

机构地区：[1]浙江大学生物科学系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2001年第2期165-168,共4页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：浙江省自然科学基金资助项目! ( 396 0 70 )

摘　　要：本项研究表明 ,嗜热厌氧纤维素降解细菌 Clostridium sp.EVA1在降解纤维素的过程中 ,菌体对不溶性纤维素底物具有强烈的粘附作用 .在透明圈中参与纤维素降解的菌体周围具有波纹状突起结构 ,并且菌体与纤维素的粘附位点是一个电子致密区域 .该菌株产生的纤维素酶是与细胞相连的胞外酶 ,培养液中无纤维素酶活性 .以脱脂棉花为碳源培养产生的纤维素酶活性最大 .该菌株纤维素酶在The intense adherence of Clostridium sp. EVA1, a thermophilic cellulolytic anaerobe, to insoluble cellulose was reported. Compared to the surface of cellobiose grown cell, wave like exocellular protuberances located on the surface of cellulose degrading cell. The attachment site of cell to cellulose was electronic dense zone. The cellulase of EVA1 was cell associated exocellulase, and no cellulase activity existed in the medium. It was shown that EVA1 strain grown in absorbent cotton produced the maximal cellulase activity and the optimum temperature for cellulase activity was 70 ℃.

关 键 词：嗜热厌氧细菌 纤维素降解菌 粘附 纤维素酶 酶活性 碳源 温度 

分 类 号：S182[农业科学—农业基础科学] S154.381