烟碱对白细胞介素-1β诱导的培养牛脑微血管内皮细胞表达α_vβ_3整合素的增强作用  

Enhancement of IL-1β-induced expression of α_vβ_3 integrin by nicotine in cultured bovine cerebral microvascular endothelial cells

作　　者：徐江平[1] 杨雪梅[1] 

机构地区：[1]第一军医大学药理学教研室,广东广州510515

出　　处：《第一军医大学学报》2001年第4期266-268,共3页Journal of First Military Medical University

基　　金：中国博士后科学基金!([98]6号)

摘　　要：目的观察烟碱对牛脑微血管内皮细胞（Bovine cerebral microvascular endothelial cells,ＢＣＭＥＣ）表达αvβ3整合素 的影响。方法离体培养新生牛脑微血管内皮细胞；血细胞计数仪测定ＢＣＭＥＣ粘附大鼠血单核细胞（ＭＮ）的数目；应用 ＥＬＩＳＡ法检测ＢＣＭＥＣ对αvβ3整合素的表达量。结果高浓度烟碱（１０－５mol/L)单独作用于ＢＣＭＥＣ２h后，使ＢＣＭＥＣ 对 ＭＮ的粘附率从（１２．９＋０．４）％增至（１５．７＋０．６）％，且 ＢＣＭＥＣ也可表达少量的 αvβ３整合素；而当烟碱与ＢＣＭＥＣ作用２ ｈ后，再经白细胞介素－１β（ＩＬ－１β）诱导４ｈ，烟碱可浓度依赖性（１０－７～１０－５mol/L）地增强ＩＬ－１β的诱导作用，增加ＢＣＭＥＣ 对ＭＮ的粘附率以及Ｅ－选择素的表达量；抗αvβ3整合素抗体（αβＡＩＡb）能显著阻断ＩＬ－１β诱导ＢＣＭＥＣ后对ＭＮ的粘 附率。结论烟碱具有增强ＩＬ－１β诱导的脑微血管内皮细胞表达αvβ３整合素的作用。Objective To study the effect of nicotine on the expression of αvβ3 integrin in cultured neonatal bovine cerebral microvascular endothelial cells (BCMEC). Methods Flow cytometry was utilized to determine the cell ratio of in vitro cultured neonatal BCMECs adhesive to rat monocytes (MN), after the former were treated with nicotine prior to the incubation with MNs. The effect of niocotine on αvβ3 expression in BCMEC was observed with ELISA. Results In response to 105 mol/L ni cotine pretreatment, the adhesive cell ratio of BCMECs to MNs was increased (P<0.05) and enhanced αvβ3, expression was observed, in comparison with those when nicotine of lower concentrations were administered. In contrast, more obvious increase in the adhesive cell ratio and αvβ3, expression was observed when the pretreatment protocol with nicotine (10-7～10－５ mol／L) and IL-１β was adopted, and the effect of nicotine was concentration-dependent. The endothelial adhesion to MNs induced by nicotine and IL-β was obviously blocked by anti-αvβ3 integrin Ah. Conclusion Nicotine may increase the expression of αvβ3 integrin in BCMEC.

关 键 词：烟碱 血管内皮 单核细胞 白细胞介素-1Β αvβ3整合素 脑血管损伤 

分 类 号：R363[医药卫生—病理学]