Expression of Prolactin Gene in Rat Prolactinoma Induced by 17-β-Estradiol  

17－β－雌二醇诱发SD大鼠催乳素瘤形成过程中催乳素基因的表达

作　　者：许荣焜[1] 郭传海[1] 黄曼影[1] 韩小四[1] 周远征[1] 单惠敏[1] 

机构地区：[1]中国医学科学院基础医学研究所生理研究室

出　　处：《Developmental and Reproductive Biology》1995年第2期46-52,共7页发育与生殖生物学报（英文版）

摘　　要：Rats were implanted subcutaneously with silastic capsules containing 10 mg 17-β-estradiol. After 30, 60 and 120 days, their pituitary weights and plasma PRL levels were found to increase significantly. The administration of β-estradiol also produced a marked rise of PRL mRNA concentrations in the rat total RNA, but the sharper rise of serum PRL levels indicates that estradiol not only promotes transcription of prolactin gene, but also improves the efficiency of translation of the transcription product.给ＳＤ大鼠皮下埋植内含１７－β－雌二醇硅橡胶管３０、６０、１２０天后，发现大鼠垂体重量随给药时间延长呈持续性增加；用放射免疫法测定血浆催乳素（ｐｒｏｌａｃｔｉｎ，ＰＲＬ）含量，亦明显依次升高；用Ｎｏｒｔｈｅｒｎ印迹杂交方法检测垂体细胞中ＰＲＬ基因，发现ＰＲＬｍＲＮＡ含量也依次有显著增加，但其增加却远低于血浆ＰＲＬ含量的增加，提示１７－β－雌二醇除了促进ＰＲＬ基因的转录外，还可能增加ＰＲＬｍＲＮＡ的翻译效率。

关 键 词：ESTRADIOL PROLACTINOMA Prolactin  Gene expression 

分 类 号：R736.4[医药卫生—肿瘤]