喉癌相关基因LCRG1的克隆和表达分析  

Cloning and Expression Analysis of a Laryngeal Carcinoma Related Gene, LCRG1

作　　者：李友军[1] 谢海龙[1] 陈主初[1] 何春梅[1] 

机构地区：[1]中南大学湘雅医学院肿瘤研究所,长沙410078

出　　处：《生物化学与生物物理学报》2001年第3期315-319,共5页

基　　金：国家自然科学基金!(No .3990 0 0 5 2 );卫生部科学研究基金!(No .98 1 118)资助项目&&

摘　　要：喉癌是严重危害人类健康的一类恶性肿瘤。选用在mRNA差异显示研究中获得的在喉癌组织中表达显著下调且代表新基因的EST之一 (AF10 0 5 6 ) ,设计引物进行cDNA文库筛选 ,得到一全长为 3 44 8bp的cDNA序列 ,其开放读码框编码 2 88个氨基酸 ,与已知蛋白质无明显同源性 ,属一新发现的基因 ,GenBank接受号为AF2 6 8387。与GenBank数据库匹配分析 ,发现该基因包含 6个外显子 ,基因组DNA跨越 6 0多个kb ,定位于染色体 17q12～ 2 1.1上。MTN膜杂交显示该基因在多组织中表达。差异RT PCR显示有 40 % (12 / 30 )的喉癌组织表达下调 ,RT PCR显示有 5 4.5 % (6 / 11)其他肿瘤细胞系表达缺失。把该基因命名为喉癌相关基因 (laryngealcarcinomarelatedgene 1,LCRG1)。By screening a human adult cDNA library using a cDNA fragment (AF10056) as a probe, which is significantly down regulated in laryngeal carcinoma and represents a novel gene, a cDNA, LCRG1 (laryngeal carcinoma related gene 1) was identified, which was significantly down regulated in 12 of 30(40%) primary laryngeal carcinomas and in 6 of 11(54.5%) various cancer cell lines. This gene, localized on chromosome band 17q12—21.1 by alignment of it with STS markers, was composed of six exons and spaned about 60 kb of genomic DNA with a 3.4 kb mature transcript. The putative protein encoded by this gene was 288 amino acid with no significant homology with any known proteins in databases. LCRG1 was expressed in many tissues, as shown by MTN blot analysis. These data suggest that LCRG 1 is related to the laryngeal carcinoma.

关 键 词：喉癌 LCRG1 表达分析 基因克隆 

分 类 号：Q78[生物学—分子生物学]