我国部分地区新城疫病毒分子流行病学研究  

The molecular epizootiology of newcastle disease viruses isolated from some regions in China

作　　者：瞿国润 吴艳涛[1] 张如宽[1] 刘秀梵[1] 

机构地区：[1]扬州大学畜牧兽医学院,江苏扬州225009

出　　处：《中国兽医科技》2001年第3期10-12,共3页Chinese Journal of Veterinary Science and Technology

基　　金：国家自然科学基金!资助项目 (398932 90 )

摘　　要：将来自江苏、浙江、上海、福建、江西和新疆 6省 (市、自治区 )的 10株新城疫病毒 (NDV)分离株经克隆纯化 ,用反转录PCR扩增其F基因 9× 10 2 bp片段 ,测定序列 ,通过与国内外已发表的NDVF基因部分序列进行比较 ,测定的 10个分离株分别属于Ⅲ、Ⅵ、Ⅶ 3个基因型。其中近年流行的基因Ⅲ型病毒与同属于该型的经典强毒F48E8的同源性较低 ,约为 92 % ;明显小于近年流行的基因Ⅲ型毒株之间的同源性 (>99% ) ;近年分离的NDVⅥ型毒株之间的同源性和Ⅶ型NDV毒株之间的同源性为 92 %— 96 %。从各毒株的分离时间来看 ,新基因型NDV毒株不断出现 ,而原有的NDV强毒也能分离到。Fusion (F) genes9×10 2bases segments (from nucleotides 6 to 889) of ten isolates of newcastle disease viruses (NDV) from Jiangsu, Zhejiang, Shanghai, Jiangxi and Xinjiang six provinces, which had been cloned and purified, were amplified by RT PCR and sequenced. By comparison of the published partial sequences of NDVs F genes, the ten isolates of NDV belonged to three distinct genotypes (Ⅲ,Ⅵ,Ⅶ). The identity between the genotype Ⅲ isolates which have been prevalent in some districts and the classical virulent strain F48E9 of NDV which belongs to genotype Ⅲ was lower and approximately 92%, remarkably lower than that (>99%) between the genotype Ⅲ isolates which were recently found. However, the homologies between both genotype Ⅵ isolates and genotype Ⅶ isolates of NDV isolated recently in the various areas ranged from 92% to 96%. It was found that the incidents caused by new subgenotype (Ⅶ) of NDV were increasing and the primary virulent genotypes (Ⅲ andⅥ) of NDV are prevalent as yet .

关 键 词：新城疫病毒 F基因 系统发育进化树 分子流行病学 地方毒株 

分 类 号：S852.659.5[农业科学—基础兽医学]