HLA-DRB1基因多态性与急性淋巴细胞白血病和慢性髓性白血病易感性关联的研究  

Association between HLA-DRB1 allele polymorphism and susceptibility to acute lymphoblastic leukemia and chronic myelogenous leukemia in Chinese Han

作　　者：曹孟德[1] 陈宗德[1] 苏堤[1] 秦东春[2] 岳保红[2] 苏天水[2] 燕桂香[2] 盛光耀 

机构地区：[1]河南医科大学微生物学与免疫学教研室 [2]河南医科大学第一附属医院检验科 [3]小儿科

出　　处：《中华微生物学和免疫学杂志》2001年第3期278-280,共3页Chinese Journal of Microbiology and Immunology

基　　金：河南省科技攻关项目!(9712 0 0 2 7)

摘　　要：目的　探讨急性淋巴细胞白血病 (ALL)和慢性髓性白血病 (CML)易感性与HLA DRB1基因多态性之间的关联性 ,找出急性淋巴细胞白血病和慢性髓性白血病的易感基因。方法　采用序列特异性引物聚合酶链反应 (PCR SSP)DNA分型技术对 5 6例ALL患者、48例CML患者和 10 5例健康对照进行了HLA DRB1基因分型。结果　ALL患者组与HLA DR7基因关联 ,基因频率为 2 4.1% ,RR=2 .5 6 ,χ2 =7.34 ,P <0 .0 1;CML患者组与HLA DR4基因关联 ,基因频率为 2 2 .9% ,RR =5 .0 76 ,χ2 =17.88,P <0 .0 1;其他等位基因频率在实验组与对照组间差异无显著性。结论　提示在河南汉族人群中 ,HLA DR7与ALL有关联 ,HLA DR4与CML有关联。Objective To explore the possible association between HLA-DRB1 allele polymorphism and susceptibility to acute lymphoblastic leukemia(ALL) as well as chronic myelogenous leukemia(CML) in Chinese He′nan Han People and to find the predisposing genes of ALL and CML. Methods 105 normal people and 56 ALL patients and 48 CML patients of He′nan Han nationality were tested for HLA-DRB1 by polymerase chain reaction with sequence specific primers(PCR-SSP). Results Compared with normal controls, the frequency of HLA-DR7 was significantly increased in ALL group with a relative risk (RR) as 2.56 (χ2 =7.34, P<0.01) and with an etiological fraction (EF) as 0.29; the frequencies of other DRB1 alleles were not significantly increased in the ALL group. The frequency of HLA-DR4 was significantly increased in CML patient group, with a relative risk (RR) as 5.076 (χ2=17.88, P<0.01) and with an etiological fraction (EF) as 0.18, while the frequencies of other DRB1 alleles were not significantly increased in CML group. Conclusion These results showed that susceptibility to ALL is positively related to HLA-DR7 gene and susceptibility to CML is strongly correlated with HLA-DR4 gene.

关 键 词：HLA-DRB1基因 分型 急性淋巴细胞白血病 慢性髓性白血病 

分 类 号：R733.71[医药卫生—肿瘤]