大蒜新素对肾型高血压大鼠和老年大鼠脑皮质微粒体ATPase活力的影响  

Effect of allitridi on the ATPase activities of cerebral cortex microsomes in renovascular hypertensive rats and senile rats

作　　者：马晓红[1] 潘鑫鑫[1] 刘天培[1] 

机构地区：[1]南京医科大学药理学教研室

出　　处：《中国药理学通报》2001年第2期205-207,共3页Chinese Pharmacological Bulletin

摘　　要：目的研究大蒜新素（ａｌｌｉｔｒｉｄｉ，Ａｌｌ）对肾型高血压大鼠和老年大鼠脑皮质微粒体ＡＴＰａｓｅ活力的影响。方法用两肾一夹法建立大鼠Ｇｏｌｄｂｌａｔｔ肾型高血压模型；以钼酸铵定磷，采用光电比色法测定ＡＴＰａｓｅ活力。结果肾型高血压大鼠和老年大鼠中，脑皮质微粒体中Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活力比成年大鼠中显著降低，Ｍｇ２＋ＡＴＰａｓｅ无明显改变。大蒜新素（１５ ｍｇ·ｋｇ．ｄ－ｌ ｉｇ， ６０ ｄ）可显著提高肾型高血压大鼠和老年大鼠脑皮质微粒体中Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活力，而对Ｍｇ２＋－ＡＴＰａｓｅ无明显影响。结论大蒜新素对大鼠脑皮质微粒体的Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活力的提高可能有利于大蒜新素防治脑缺血作用。AIM To investigate the effect of allitridi on the ATPase activities of cerebral cortex microsomes in two-kidney one-clip renovascular hyper- tensive and senile rats, which are prone to the cerebral ischemia. METHODS Cerebral cortex microsomes were purified from renohypertensive and senile Sprague-Dawley rat. ATPase activities were determined according to inorganic phosphate measurement. RESULTS Allitridi (15 mg·kg-1 ig, 60 d) could significantly improve the Na+, K+ -ATPase and Ca2+, Mad+ -ATPase activities of brain cortex microsomes in two-kidney one-clip revohypertensive rats and senile rats, while had no obvious influences on Mg2+ -ATPase activity. CONCLUTION The influences of allitridi on the ATPase activities of brain in renovascular hypertensive and senile rats are benefit to its prevention and therapy to cerebral ischemic injury.

关 键 词：脑缺血 肾型高血压 大鼠 大蒜新素 脑皮质微粒体 ATPase活力 

分 类 号：R544.14[医药卫生—心血管疾病] R285.5[医药卫生—内科学]