用SDS—PAGE进行鸡败血霉形体结构蛋白分析  

ANALYSIS OF STRUCTURAL PROTEINS OF MYCOPLASMA GALLISEPTICUM BY SODIUM DODECYL SULFATE—POLYACRYLAMIDE GEL ELECTROPHORESIS

作　　者：于圣青[1] 刘晓文[1] 徐步[1] 丁铲[1] 

机构地区：[1]江苏省家禽科学研究所,扬州225003

出　　处：《畜牧兽医学报》2001年第3期249-252,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：江苏省"九.五"重点攻关课题!(BE96 5 0 2 )

摘　　要：利用SDS—PAGE方法对禽败血霉形体R、S6、F株和 6株国内分离株进行了结构蛋白比较分析 ,电泳凝胶染色扫描结果显示 ,各株之间结构蛋白差异较大。R、S6株 p6 4蛋白表达量较高 ,D96 0 4株与其相似性较高。D96 0 1、D96 0 3和D96 0 5与F株均有一条特异的 75kDa条带 ,提示我国分离的MG强毒株可能存在着多样性。Through the sodium dodecyl sulfate—polyacrylamide gel electrophoresis we analysed and compared the structural proteins of 9 Mycoplasma gallisepticum strains,including type strain S6,F,and 6 field isolates from China.The result showed that the protein profiles of all these strains were different,the p64 was expressed in higher amounts in vitro in MG strains considered more virulent (S6 and R) than in MG strains of reduced virulence (F,et al).Protein profiles showed similarities of S6 and R.F,D9601,D9603 and D9605 all had a special 75 kDa band,but all virulent strains had not.The result suggests that the virulent field isolates in China maybe diversified.

关 键 词：SDS-PAGE 鸡 败血霉形体 结构蛋白 慢性呼吸道病 粘附机理 

分 类 号：S858.31[农业科学—临床兽医学] S852.62[农业科学—兽医学]