异戊二烯/1,2-环氧己烷嵌段共聚物的合成及其固定化酶  

Synthesis of Block Copolymer of Isoprene and 1,2-Epoxyhexane and Its Application to Immobilizing Enzyme

作　　者：胡志国[1] 张一烽[1] 薛怀国[1] 沈之荃[1] 

机构地区：[1]浙江大学高分子研究所,杭州310027

出　　处：《应用化学》2001年第5期373-376,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金 !(2 99740 2 4);国家重点基础研究! (G19990 6 480 1)

摘　　要：采用三元催化剂 [Nd( P2 0 4) 3-Al( i-Bu) 3-Br CH2 CH2 Br]以顺序聚合法合成异戊二烯 / 1 ,2 -环氧己烷嵌段共聚物 ,研究了共聚合特征 .用红外光谱和元素分析对共聚物进行表征 .用电化学吸附法将葡萄糖氧化酶固定在该共聚物中制成异戊二烯 / 1 ,2 -环氧己烷嵌段共聚物葡萄糖氧化酶电极 ,该电极呈现典型的酶催化反应动力学特征 .固定葡萄糖氧化酶的表观米氏常数为 32 .2 mmol/ L,酶催化反应的活化能为 37.2 k J/The block copolymer of isoprene(Ip) and 1,2-epoxyhexane(HEO) was synthesized by rare earth coordination catalyst, [Nd(P 204 ) 3-Al( i -Bu) 3-BrCH 2CH 2Br]. The polymerization behavior was studied in detail. The polymer was characterized by IR and elemental analysis. The poly(isoprene- b -1,2-epoxyhexane) glucose oxidase electrode, which was formed by immobilization of glucose oxidase on the poly(Ip- b -HEO) film using the electrochemical method, has kinetic characteristics of the typical enzyme-catalyzed reaction. The apparent Michaelis constant and activation energy for the immobilized glucose oxidase are 32 2 mmol/L and 37 2 kJ/mol, respectively.

关 键 词：稀土催化剂 异戊二烯 嵌段共聚 葡萄糖氧化酶 固定化酶 1 2-环氧己烷 电极 催化活性 催化合成 

分 类 号：O632[理学—高分子化学] Q554[理学—化学]