毛细管胶束电动色谱法测定血管紧张素转化酶的活性  

Determination of Angiotensin-Converting Enzyme Activity by Micellar Electrokinetic Capillary Chromatography

作　　者：徐小华[1] 张蓉真[1] 盛思梅[1] 陈天豹[1] 李珑[1] 饶平凡[1] 

机构地区：[1]福州大学生物工程研究所,福建福州350002

出　　处：《色谱》2001年第1期68-70,共3页Chinese Journal of Chromatography

基　　金：福建省 2 11重点学科仪器资助! (No .2 15 15 )

摘　　要：建立了应用毛细管胶束电动色谱 (MECC)灵敏、快速的测定血管紧张素转化酶 (ACE)活性的方法。通过对电压、上样时间、电极缓冲液体系等影响因素的优化 ,探讨了方法的可行性 ,确立了最佳测定条件 (电压 :8 1kV ;上样时间 :1s;电极缓冲液 :2 0mmol/L硼酸盐缓冲液 (pH 9 0 ,含 5 0mmol/LSDS) ;检测波长 :2 2 8nm)。方法的最低ACE活性检测限为 5pmol/min(以 2倍的信噪比计 )。A sensitive and rapid method was developed for angiotensin converting enzyme (ACE) activity determination by micellar electrokinetic capillary chromatography (MECC) MECC was carried out to separate and quantify the products of the enzymatic reaction using Hip Leu His as the substrate in 20 mmol/L boric acid borate buffer (pH 9 0) including 50 mmol/L SDS as the run buffer at an applied voltage of 8 1 kV The electrophoresis was monitored at 228 nm, and completed in 6 minutes The detection limits of ACE activity was 5 pmol/min(signal to noise ratio was 2).

关 键 词：毛细管胶束电动色谱法 血管紧张素转化酶 酶活性测定 血清 疾病诊断 

分 类 号：R446.11[医药卫生—诊断学] Q556.3[医药卫生—临床医学]