底物诱导和分批补料对巨大芽孢杆菌环氧化物水解酶生物合成的影响  

Effect of Substrate Induction and Fed-batch Operation on Biosynthesis of Epoxide Hydrolase from Bacillus megaterium ECU1001

作　　者：朱智东[1] 唐燕发[1] 许建和[1] 叶勤[1] 储炬[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2001年第3期243-246,共4页Journal of East China University of Science and Technology

基　　金：荷兰 DSM公司委托研究项目 !(ACER 980 8-0 12 6)

摘　　要：从土壤中筛选得到的巨大芽孢杆菌 ( Bacillus megaterium) ECU1 0 0 1所产环氧化物水解酶能高度对映选择性地水解外消旋缩水甘油苯基醚。在摇瓶和 5L发酵罐中培养时初始产酶水平分别为 1 1 .0 U/L和 31 .0 U/L。通过添加底物诱导 ,可使摇瓶培养中酶量达到 61 .0 U/L;在 5L发酵罐中通过补料分批发酵 ,酶活可进一步提高到 96.An epoxide hydrolase biosynthesized by Bacillus megaterium EUC1001 isolated from soil can hydrolyze racemic phenyl glycidyl ether (PGE) with high enantioselectivity. Initial activities of the epoxide hydrolase obtained in flask and 5L fermentor were 11.0U/L and 31.0U/L, respectively. The enzyme activity in flask reached 61.0U/L by adding the substrate as an inducer and it could even reach 96.6U/L through fed batch fermentation in a 5L fermentor.

关 键 词：缩水甘油苯基醚 环氧化物水解酶 巨大芽孢杆菌 底物诱导 分批补料发酵 产酶水平 生物合成 

分 类 号：TQ925[轻工技术与工程—发酵工程]