尿多酸肽对人肝癌细胞Smmu7721体内体外抑制作用  

Uroacitide from Human Urine Against Human Liver Cancer Cell Smmu 7721 in vitro and in vivo

作　　者：李湛君[1] 常慧兰[1] 徐康森[1] 张义兴 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]合肥永生制药公司,231202

出　　处：《药物分析杂志》2001年第3期185-188,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的 :研究由人尿提取的尿多酸肽 (uroacitide)对人肝癌细胞的体内体外抑制作用。方法 :体外实验 ,以四唑盐(MTT)比色法测定培养板中加入尿多酸肽后 ,人肝癌细胞Smmu772 137℃ 5 %CO2 培养箱培养 72h的生物活性 ;体内抑瘤实验为裸鼠皮下接种人肝癌细胞Smmu 772 1,待肿瘤生长后 ,连续每天ip尿多酸肽 10天 ,观察其抑制作用。结果 :体外实验 ,尿多酸肽剂量为 0 0 3～ 2 0 0mg·mL- 1 ,对人肝癌细胞Smmu 772 1的IC5 0 为 0 35～ 0 49mg·mL- 1 ;体内实验 ,尿多酸肽剂量 5 0 0～ 2 0 0 0mg·kg- 1 ,对裸鼠接种人肝癌细胞Smmu 772 1的抑瘤率在 38 9%～ 6 7 9%。结论 :尿多酸肽对人肝癌细胞Smmu772 1体内体外均有显著的抑制作用。Objective:To study the inhibitory effect of Uroacitide which is a mixture isolated and purified from human urine on human liver cancer cells in vitro and in vivo.Methods:in vitro study,MTT assay was used to determine the bioassay activity of human liver cancer cells Smmu 7721 after adding Uroacitide in incubating at 37 ℃ and 5% CO 2 for 72h.In vivo study,nude mice were injected sc with human liver cancer cells Smmu 7721,after tumor growing daily ip with Uroacitide for 10 day to observe the inhibitory effect.Results:In vitro Uroacitide at concentration in the range of 0.03～2.00 mg·mL -1,the IC 50 were 0.35～0.49 mg·mL -1.In vivo,Uroacitide of 500～2 000 mg·kg -1 can suppress the growith of human liver cancer cells Smmu 7721 in nude mice,the inhibitory rate were 38.9%～69.7%.Conclusion:Uroacitide show significant suppressing action on the growth of human liver cancer cells in vitro and in vivo.

关 键 词：尿多酸肽 人尿 人肝癌细胞 抑制作用 

分 类 号：R735.7[医药卫生—肿瘤]