Smad2、3在人牙髓组织中的表达及意义  

The expression of Smad2 and 3 proteins in human dental pulp tissue

作　　者：包柳郁[1] 牛忠英[1] 陈健[1] 何文喜[1] 臧晓霞[1] 

机构地区：[1]西安第四军医大学口腔医学院,710032

出　　处：《实用口腔医学杂志》2001年第3期182-184,共3页Journal of Practical Stomatology

基　　金：国家自然科学基金资助项目!编号 :39870 789

摘　　要：目的 :观察转化生长因子β特异的细胞内信号转导分子 Smad2、3在人牙髓组织中的表达及其变化 ,探讨 Smad2、3在牙髓损伤修复过程中的作用。方法 :用免疫组化方法检测 Smad2、3在正常、龋坏及炎症牙髓组织中的表达。结果 :Smad2 ,3在正常和龋坏牙髓的成牙本质细胞层呈强阳性表达 ,在下方的多细胞区及中心部牙髓有阳性或弱阳性表达 ,炎症牙髓浸润的炎细胞中 Smad2、3呈强阳性表达 ,各类牙髓中 Smad2的表达较 Smad3略强。结论 :Smad2、3在正常、龋坏及炎症牙髓组织中有表达 ,提示 Smad2、3可能在 TGF-β调节牙髓细胞增殖、分化的信号转导途径中发挥一定的作用。?Objective:To investigate the expression of Smad2 and 3 proteins in human dental pulp tissue and to study the effect of Smad2 and 3 on dental pulp repair after injury. Methods: Immunohistochemical technique was applied to detect the expression and distribution of Smad2 and 3 proteins in human normal (15 cases) ,carious (20 cases) and inflammatory(10 cases) pulp tissue. Results: The odontoblasts of normal and carious teeth were strongly stained by smad 2 and 3 antibodies, weaker staining was observed in the underlying cell rich layer and pulp core, strong specific staining was detected in inflammatory cells at the sites of pulp inflammation. Expression of Smad2 was higher than that of Smad3 in all specimens . Conclusion: Smad2 and 3 might play an important role in the intracellular signaling pathway of TGF β modulating the proliferation and differentiation of dental pulp cells.

关 键 词：SMAD2 Smad23 牙髓 表达 免疫组织化学 

分 类 号：R780.2[医药卫生—口腔医学]