鸦胆子油乳对膀胱癌影响的实验研究  

An experimental study of brucea javanica oil emulsion on bladder carcinoma

作　　者：刘悦[1] 王禾[2] 符庆吉[1] 王鹏飞[2] 郑洪柱[1] 苏辉[1] 黄亮[1] 

机构地区：[1]解放军第二一零医院泌尿外科,大连116021 [2]西安第四军医大学西京医院泌尿外科

出　　处：《中华泌尿外科杂志》2001年第6期336-338,共3页Chinese Journal of Urology

摘　　要：目的　探讨鸦胆子油乳注射液 (简称鸦油乳 )抑制膀胱癌的作用机制。　方法　将不同浓度的鸦油乳加入体外培养的BIU 87细胞 ,观察对BIU 87细胞生长、组织结构及细胞周期的影响 ;对亚硝胺 (BBN)诱导膀胱癌的ICR小鼠行鸦油乳膀胱灌注 ,光镜和电镜观察对膀胱癌的抑制作用。　结果　鸦油乳对BIU 87细胞生长有明显的抑制作用 ,可直接破坏膀胱癌细胞膜、线粒体膜、内质网膜及核膜等膜性系统 ,使膀胱癌细胞变性并坏死 ;鸦油乳可阻止BIU 87细胞由G0 /G1期向S期进展 ,抑制DNA合成。鸦油乳膀胱灌注对BBN诱导的膀胱癌早期病变有明显的抑制作用。　结论　鸦油乳对膀胱癌细胞有明显的抑制作用 。Objective To study the inhibition effect of brucea javanica oil emulsion on bladder carcinoma. Methods Using electron microscopy and flow cytometry,the effects of brucea javanica oil emulsion on human bladder carcinoma BIU 87 cell line in vitro and in BBN induced bladder carcinoma in ICR mice were studied. Results Significant growth suppression of human bladder carcinoma BIU 87 cell line and BBN induced bladder carcinoma in ICR mice was observed with the use of brucea javanica oil emulsion.Carcinoma cells retrogressed with brucea javanica oil emulsion being attacked directly on the plasmalemma system such as mitochondria and endoplasmic reticula.Remarkable G0/G1 phase cell accumulation and decrease in proportion of S phase cell were observed by flow cytometry after the drug administration.The DNA content diminished. Conclusions Brucea javanica oil emulsion,a traditional chinese medicine,can inhibit bladder carcinoma cell and might be helpful to the clinical treatment and prevention of bladder carcinoma.

关 键 词：膀胱肿瘤 膀胱癌 鸦胆子油乳 实验研究 

分 类 号：R737.14[医药卫生—肿瘤]