DPA法检测细胞凋亡及其在白血病中的应用  

Analysising Apoptosis by Diphenylamine and Its Use in Leukemia

作　　者：何维敬[1] 张义[1] 毕正[1] 张建业[2] 李登欣[2] 

机构地区：[1]山东大学齐鲁医院,山东济南250012 [2]山东大学西校区,山东济南250012

出　　处：《陕西医学检验》2001年第2期6-7,共2页Shaanxi Journal of Medical Laboratory Sciences

摘　　要：目的　建立一种检测白血病细胞凋亡的新方法。方法　高速离心将 DNA样品区分为小分子量DNA片段及高分子量 DNA(分别来自凋亡和未凋亡细胞 )两部分 ,二苯胺 (DPA)法测定各部分 DNA含量 ,计算 DNA片段率 ,从而分析细胞凋亡情况。以此方法分析了白血病细胞株 HL- 60受不同剂量抗癌药物 VP- 1 6诱导时的凋亡情况。结果　与 FCM法相比 ,各剂量组细胞凋亡率无显著差别 (P>0 .0 5) ,经密度梯度离心法分离提取的凋亡细胞 ,两种方法测得细胞凋亡率亦无明显差别 (P>0 .0 5)。结论　二苯胺法测定Aim To explore a new method to analysis leukemic cells apoptosis.Method Dependenting the characteristic of apoptosis cleavage of DNA into internucleosomal fragments,low and high molecular weight DNA(DNA from apoptotic and non apoptotic cells)were separated by high speed centrifugation,which measured by diphenylamine(DPA)reaction.The percent of DNA fragments was been evaluated the percent of apoptotic cells of total.Use this method,we analysed the apoptotic effect of HL 60 cells induced by Etoposide.Results No significant change ( P >0 05)was found in the percent of apoptotic cells induced by different concentration of Etoposide measured by this method and by FCM method,and the same results was also detected in the separated apoptotic cells.Conclusion Measuring DNA fragments by diphenylamine is a simple method to analysis leukemic cells apoptosis.

关 键 词：细胞凋亡 HL-60细胞 脱氧核糖核酸片段 二苯胺 白血病 

分 类 号：R733.7[医药卫生—肿瘤]