流体切应力和Ox-LDL对内皮细胞表面ICAM-1表达的影响  

Fluid Shear Stress and Ox-LDL Modulate Surface Expression of Intercellular Adhesion Molecule-1(ICAM-1)by Endothelial Cells

作　　者：李宏妹[1] 邢海燕[1] 马丽[1] 朱文云 金永娟 

机构地区：[1]中国医学科学院,中国协和医科大学血液学研究所,邮政编码天津300020

出　　处：《微循环学杂志》2001年第2期25-26,共2页Chinese Journal of Microcirculation

基　　金：国家自然科学基金资助项目 ( 39770 2 0 4)

摘　　要：目的 :研究动脉粥样硬化 (AS)的危险因素高脂血症、流体切应力及二者共同作用对血管内皮细胞细胞间粘附分子 1(ICAM 1)表达的影响。方法 :以人脐静脉内皮细胞 (HUVECs)为研究对象 ,应用免疫组化ACB方法 ,观测Ox LDL、流体切应力及二者共同作用对HU VECs的粘附分子ICAM 1表达的影响。结果 :与对照组相比 ,( 1)Ox LDL显著增加HUVECs表面ICAM 1表达 ;( 2 )流体切应力使HU VECs表面ICAM 1表达增加 ,但ICAM 1的表达随时间依赖性增加的趋势不明显 ;( 3)共同作用后 ,HUVECsICAM 1的表达较基础表达显著增加。结论 :Ox LDL、流体切应力的改变及二者共同作用显著增加HUVECs表面ICAM 1表达。Objective: To study the effects of Ox LDL,fluid shear stress and the combination of the two stimuli on the expression of ICAM 1 by the endothelia cells.Method: After exposure of the human umbical vein endothelial cells(HUVECs)to the shear stress of 0.72 Pa for different time,the Ox LDL of different concentration for 24 h,or the both stimuli,ICAM 1 was determined by using immunohistochemistry ABC.Results: Compared with the control,(1)after the incubation of HUVECs with Ox LDL,the expression of ICAM 1 was markedly increased;(2)application of the same fluid shear stress for different time enhanced the expression of ICAM 1 significantly,but the increase of ICAM 1 was not apparently time dependent;(3)after the both two stimuli were used,the ICAM 1 expression of HUVECs was remarkbly increased.Conclusion: Ox LDL,the change of shear stress of the combination of the two stimuli can increase the expression of ICAM 1 on the surface of HUVECs.

关 键 词：流体切应力 OX-LDL 内皮细胞 表达 ICAM-1 

分 类 号：R364.1[医药卫生—病理学]