精子发生障碍患者精蛋白基因5′端及3′端核基质结合区的研究  

The study on matrix attachment region (MAR) at 5 prime and 3 prime end of protamime genes

作　　者：李建平 张思仲[2] 肖翠英[2] 

机构地区：[1]成都市计划生育技术指导所,成都610031 [2]华西医科大学附一院医学遗传室

出　　处：《中国男科学杂志》2001年第2期82-84,共3页Chinese Journal of Andrology

摘　　要：运用PCR、PCR -SSCP、PCR产物直接测序等方法对 6 1例少精子症及无精子症患者的精蛋白基因 5′端及 3′端核基质结合区 (Matrixattachmentregion ,MAR)进行了突变筛查。结果发现 2例少精子患者在 5′端核基质结合区存在PCR -SSCP带型变异 。Mutational search at 5 prime terminus and 3 prime terminus matrix attachment region (MAR) of human protamine genes was performed by using PCR,PCR?SSCP and direct PCR produts sequencing.The results showed that 2 out of 61 oligo azoospermia patients had a different PCR-SSCP band pattern.Direct sequencing of the PCR products found that there is a heterozygous change at a nucleotide position in the sequence.

关 键 词：糖蛋白基因 核基质结合区 基因突变 少精子症 无精子症 

分 类 号：R698.2[医药卫生—泌尿科学]