大肠癌肝转移肿瘤中端粒酶活性和p16基因纯合缺失  

The telomerase activity and the homozygous deletions of p16 gene in liver metastases of colorectal carcinoma

作　　者：马晋平[1] 詹文华[1] 许多荣[2] 高劲松[3] 彭俊生[1] 何裕隆[1] 汪建平[1] 

机构地区：[1]广州中山医科大学附属第一医院胃肠外科,510080 [2]广州中山医科大学附属第一医院血液科,510080 [3]中山医科大学达安基因中心

出　　处：《中华普通外科杂志》2001年第6期365-367,共3页Chinese Journal of General Surgery

基　　金：中山医科大学"2 11工程"重点学科建设基金!资助项目( 980 97)

摘　　要：目的　研究人大肠癌肝转移肿瘤中端粒酶活性和p16基因纯合缺失及其临床意义。方法　采用TRAP银染方法和半定量多重PCR检测 2 4例大肠癌肝转移肿瘤组织中端粒酶活性及p16基因的纯合缺失情况 ,并结合临床病理参数 ,进行统计学分析。结果　本组 2 4例大肠癌肝转移肿瘤组织中检测到 19例端粒酶阳性 ,阳性率为 79 2 %。端粒酶活性与转移瘤大小、肝内转移灶数目、分化程度、HBsAg以及是否伴有肝纤维化无关。在 2 4例转移瘤组织中有 9例标本中检测到p16基因的缺失 ,阳性率为 37 5 % ,p16基因的缺失与端粒酶活性相关。结论　大肠癌肝转移肿瘤中端粒酶活性和p16基因异常对阐明大肠癌转移的生物学行为有重要的意义。Objective To investigate the telomerase activity and abnormality of p16 gene in liver metastatic tumors of colorectal carcinoma. Methods Telomerase activity was detected by a non isotopic PCR based TRAP assay (Telomeric repeat amplification protocol) and the homozygous deletions of p16 gene was detected by a semiquantitative multiple PCR in tissue samples from 24 liver metastases of colorectal carcinoma. Results Telomerase activity were observed in 19 (79 2%) out of the 24 colorectal carcinoma patients. There was no correlation hetween the telomerase activity, the number of metastasis, differentiation, liver fibrosis and HBsAg status of the patients. Homozygous deletions of p16 gene were found in 9 out of 24 (37 5%) patients, and homozygous deletions of p16 gene was significantly correlated with telomerase activity.Conclusion The abnormality in telomerase activity and homozygous deletions of p16 gene may be important for elucidating in liver metastases of colorectal carcinoma.

关 键 词：肝转移 端粒 末端转移酶 P16基因 大肠癌 

分 类 号：R735.34[医药卫生—肿瘤]