集胞藻6803在封闭式光生物反应器中的混合营养培养  

Mixotrophic Cultivation of Synechocystis sp. PCC 6803 in an Enclosed Photobioreactor

作　　者：王永红[1] 李元广[1] 施定基[2] 沈国敏[1] 茹炳根[3] 张嗣良[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237 [2]中国科学院植物研究所光合作用研究中心,北京100093 [3]北京大学生命科学学院,北京100871

出　　处：《高技术通讯》2001年第5期28-30,共3页Chinese High Technology Letters

基　　金：863计划 ( 863 819 Q 0 1);国家自然科学基金 ( 2 98760 10 )资助项目

摘　　要：采用摇瓶和封闭式光生物反应器进行了蓝藻基因工程常用宿主系统集胞藻Syne chocystis680 3的混合营养培养研究。在摇瓶中对集胞藻 680 3利用有机碳源特性进行研究的结果表明 ,光照是集胞藻 680 3利用葡萄糖的必需条件 ,混合营养培养是集胞藻 680 3的最佳培养方式。以葡萄糖为基质 ,在 2 5L封闭式光生物反应器中经过 58.5h的混合营养培养 ,集胞藻 680 3的藻细胞密度和平均生长速率分别达到 2 .50g/L和 1 .0 1 g/L/d,分别是光自养培养同期的 9倍和 1 1倍 ,这表明封闭式光生物反应器混合营养培养方式在促进集胞藻 680 3生长上有显著作用。Studies on the mixortophic cultivation of Synechocystis sp. PCC6803 in shake flasks and a 2.5L enclosed photobioreactor were carried out. The results from mixotrophic cultivations in shake flasks showed that light was necessary for mixotrophic growth of Synechocystis sp. PCC6803; compared with sucrose, glucose was a better kind of substance for Synechocystis sp. PCC6803 mixotrophically growing. In 2.5L enclosed photobioreactor, after 58.5h mixotrophic cultivation, 2.50g/L cell density and 1.01g/L/d were achieved, which were respectively 9 times and 11 times as much as those in photoautotrophic cultivation. On the basis of above results, it was concluded that mixotrophic cultivation system had an advantage over photoautotrophic cultivation system.

关 键 词：集胞藻6803 混合营养培养 光生物反应器 葡萄糖 蓝藻 

分 类 号：Q943[生物学—植物学]