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作 者:王树桐[1] 王晓燕[2] 刘均玲[1] 曹克强[1]
机构地区:[1]河北农业大学植物保护学院,河北保定071001 [2]河北农业大学生物技术中心,河北保定071001
出 处:《河北农业大学学报》2001年第2期101-107,共7页Journal of Hebei Agricultural University
摘 要:将来源于 44科的 88种中草药等植物材料的提取物稀释 5 0 0倍后 ,与马铃薯晚疫病菌 (Phytophthorainfestans)的孢子囊悬浮液混合。有 31种材料的提取物在该浓度下表现了对马铃薯晚疫病菌的游动孢子囊间接萌发的完全抑制作用 ,有 46种提取物也表现了不同程度的抑制作用 ,另有 11种提取物在该浓度下没有表现抑制作用。在抑制菌丝生长作用的试验中 ,植物提取物用黑麦培养基稀释 2 0 0倍 ,然后倒皿 ,在 8.5cm直径的培养皿中央放一马铃薯晚疫病菌菌片 ,16℃下培养 ,15d后测量菌落直径大小。结果表明 ,在供试的 83种提取物中 ,在稀释 2 0 0倍浓度下 ,有 32种提取物表现了对菌丝生长的完全抑制作用 ,2 4种表现了比较强的抑制作用 ,而有 2 7种提取物没有表现对菌丝生长的抑制作用 ,甚至还表现了对菌丝生长的促进作用。同时 ,还有Plant extracts of 88 species from 44 families of plants were diluted to 500 times, and mixed with sporangia suspension of P. infestans , then the sporangia suspensions were put into 10℃ incubator for 3 hours. The result is as follows: 31 species were completely inhibitory, 46 species were strongly or moderately inhibitory and 11 were little inhibitory to sporangia indirect germination of P. infestans . And among 83 species in 43 families of plants tested, mother extracts from 32 species were completely inhibitory, 24 were strongly inhibitory and 27 were weakly or even no inhibitory to mycelial growth of P. infestans at 200 times. There are also 19 mother extracts were not only completely inhibitory to germination of sporangia but also completely inhibitory to mycelial growth of P. infestans .
关 键 词:晚疫病菌 植物提取物 杀真菌毒性 筛选 中草药 马铃薯
分 类 号:S435.32[农业科学—农业昆虫与害虫防治] S482.292[农业科学—植物保护]
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