BT型杂交粳稻育性及其三系的若干蛋白质标记(英文)  

Protein Markers of BT Type Hybrid and Its Three Lines in Japonica Rice(Oryxa sativa L)

作　　者：洪德林[1] 佐藤光[2] 熊丸敏博[2] 曲乐庆[1] U S SADAR 

机构地区：[1]南京农业大学农学系,江苏南京210095 [2]九州大学农学部

出　　处：《中国水稻科学》2001年第3期165-168,共4页Chinese Journal of Rice Science

摘　　要：用十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS- PAGE)方法 ,对粳稻 BT细胞质雄性不育系六千辛 A,保持系六千辛 B,恢复系六千辛 R、7730 2 - 1,以及杂交组合六千辛 A/7730 2 - 1的 F1 和 F2 种子的胚乳贮藏蛋白进行了分析。结果表明 ,在谷蛋白α- 3区域 ,恢复系有两条带α- 3a和α- 3b,而六千辛 A和六千辛 B只有一条带α- 3。 F2 代具有α- 3的种子和具有α- 3a加α-3b的种子 1∶ 1分离。谷蛋白α- 4带的移动速率 ,恢复系比六千辛 A快。把较快的α- 4带记为α- 4f。 F2 代具有α- 4的种子和具有 α- 4加 α- 4f的种子也是 1∶ 1分离 ,与配子体不育类型的 F2 代花粉育性恢复基因分离比一致。系谱分析表明六千辛 R中 α-3a和α- 3b来源于 IR8。六千辛 A比六千辛Seeds of CMS line Liuqianxin A with BT cytoplasm, maintainer line Liuqianxin B, restorer lines Liuqianxin R, 77302 1, F 1 and F 2 of Liuqianxin A/77302 1 in japonica rice were analyzed for endosperm storage proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Results showed that in glutelin α 3 area, restorer lines had two bands α 3a and α 3b while Liuqianxin A and Liuqianxin B had only one band α 3. In F 2 generation, seeds with α 3 and seeds with α 3a plus α 3b segregated into 1∶1 ratio. Moving rate of glutelin α 4 band in restorer lines was faster than that in Liu qianxin A. The faster α 4 was designated as α 4f. Seeds with α 4 and seeds with α 4 plus α 4f also segregated into 1∶1 ratio in F 2, which are consistent with the segregation of restorer gene of gametophytic type. Pedigree analysis indicates that the bands α 3a and α 3b in Liuqianxin R derived from IR8. Prolamin in Liuqianxin A is easier to extract than that in Liuqianxin B.

关 键 词：粳稻 恢复系 细胞质雄性不育性 蛋白质标记 杂交水稻 谷蛋白 醇溶蛋白 

分 类 号：Q946[生物学—植物学]