表皮生长因子受体(EGF-R)放射受体分析方法(RRA)的建立  

Establishment of Radioreceptor Analysis (RRA) for Receptor of Epidermal Growth Factor (EGF-R)

作　　者：詹瑧[1] 吴慧平[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210029

出　　处：《南京中医药大学学报》2001年第4期239-241,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金 (3 0 0 70 910 )

摘　　要：目的　建立表皮生长因子受体 (EGF R)放射受体分析方法 (RRA) ,用于研究舌苔、EGF R与肿瘤的关系。方法放射配基结合法 ,12 5I标记EGF与肿瘤细胞膜EGF R结合。结果　从饱和曲线和Scatchard作图得知 1 50fmol浓度的12 5IEGF可使受体基本达到饱和 ,最大结合位点Bmax为 5.89fmol,KD为 1 0 6.3 8fmol。结论　建立的EGF RRRA方法符合实验要求 。OBJECTIVE To establish radioreceptor analysis for the receptor of epidermal growth factor to be used in the investigation of the relationship between the tongue coating, EGF R and tumor. METHOD Radioligand method was used to combine 125 I labeled EGF with tumor cell membrane EGF R. RESULT Saturation curve and Scatchard graph showed that 125 I EGF at the concentration of 150 fmol could bring about the saturation of the receptor, with Bmax being 5.89 fmol and KD being 106.38 fmol. CONCLUSION The establishment of EGF R RRA method meets the requirements of experiment and the method can be used in the investigation of the tongue coating.

关 键 词：表皮生长因子受体 放射受体分析方法 舌苔研究 肿瘤 

分 类 号：R730.2[医药卫生—肿瘤]