反义c-myc重组腺病毒对人肝细胞癌细胞的实验性治疗  

Experimental therapy of adenovirus-transfered antisense c-myc on hepatocellular cancer cell

作　　者：张海增[1] 林晨[1] 邵永孚[1] 隗月[2] 余昌中[2] 付明[2] 张雪艳[2] 梁肖[3,4] 吴旻[2] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院腹部外科,北京100021 [2]中国医学科学院分子肿瘤学国家重点实验室,北京100021 [3]中国医学科学院,北京100021 [4]分子肿瘤学国家重点实验室

出　　处：《中华医学杂志》2001年第11期673-676,共4页National Medical Journal of China

基　　金：国家 8 6 3高科技研究发展计划基金资助项目 (Z 2 0 0 1 0 2)

摘　　要：目的　研究携带反义c myc的重组腺病毒 (Ad Asmyc)对人肝细胞癌细胞体外、体内的治疗作用及其机制。方法　用Ad Asmyc感染人肝细胞癌细胞系BEL 740 2和QSG 770 1,绘制细胞生长曲线 ,进行克隆形成实验 ,研究Ad Asmyc在体外的抗瘤作用 ,在瘤内注射Ad Asmyc,观察裸鼠皮下移植瘤的生长情况。应用逆转录聚合酶链反应 (RT PCR)及Westernblot检测相关基因表达 ,应用流式细胞术、梯形DNA、DNA原位末端标记研究细胞周期及细胞凋亡情况。结果　感染Ad Asmyc后 ,BEL 740 2及QSG 770 1细胞内c myc基因表达降低 ,肿瘤细胞的生长受到明显抑制 ,而且出现了G1期阻滞及凋亡 ,体内注射Ad Asmyc后裸鼠皮下移植瘤的生长也得到明显抑制。结论　Ad Asmyc在体外及体内可使肝细胞癌出现细胞生长抑制 ,发生凋亡 ,具有明显的抗癌作用 ,是有应用前景的抗癌药物。Objective To investigate the therapeutic effects of Adenovirus transfered antisense c myc (Ad Asmyc) on hepatocellular cancer cells and its mechanism. Methods Human hepatocellular cancer cell line BEL 7402 and QSG 7701 were transfected with Ad Asmyc. Cell growth curve and coloning formation were used to investigate the therapeutic effects of Ad Asmyc in Vitro. The treating effects of Ad Asmyc on transplanted tumor in nude mice was also studied. RT PCR and Western blot were used to detect relating gene expression. Flowcytometry, DNA ladder and tunel assay were used to study the cell cycle and apoptosis. Results The expression of c myc in cancer cells was reduced after infected Ad Asmyc. Ad Asmyc had obviously inhibiting effectes on the growth of two hepatocellular carcinoma cell lines in vitro and also in vivo. It can induced cell cycle arrest (G1 arrest) and apoptosis. Conclusion Ad Asmyc can inhibit HCC cell proliferation and induce apoptosis, so has obvious therepatuic effects on hepatocellular cancer cells.

关 键 词：重组腺病毒载体 基因疗法 肝细胞癌 反义C-MYC 

分 类 号：R735.7[医药卫生—肿瘤] R730.54[医药卫生—临床医学]