人类白细胞抗原-DRB1与胃腺癌及其临床特征和幽门螺杆菌感染的相关性  

Study on association of HLA-DRB1 alleles with gastric carcinoma and Helicobacter pylori infection

作　　者：林军[1] 邓长生[1] 孙洁[1] 熊平[2] 汪亚平[3] 肖建群[1] 

机构地区：[1]武汉大学中南医院消化内科 [2]华中科技大学同济医学院免疫学教研室 [3]中国科学院水生生物研究所国家重点实验室

出　　处：《中华消化杂志》2001年第3期165-167,共3页Chinese Journal of Digestion

摘　　要：目的　探讨人类白细胞抗原 (HLA) DRB1等位基因与胃腺癌及其临床特征和幽门螺杆菌(Hp)感染的关联性。方法　运用序列特异性引物聚合酶链反应和等位基因序列分析技术 ,检测无亲缘关系湖北省汉族健康人 136例、胃癌组 6 3例的HLA DRB1基因。内镜活检、Giemsa染色和 (或 )外周血ELISA检查胃黏膜Hp感染情况。SAS软件数据处理。 结果　HLA DRB10 90 1、12等位基因均与湖北省汉族人胃腺癌呈正相关 ;HLA DRB115等位基因则呈负相关。携带及非携带上述各等位基因患者 ,分别就其临床特征 ,包括平均患病年龄、性别比、肿瘤原发部位、TNM分期、肿瘤细胞分化程度以及Hp感染率等情况比较 ,差异均无显著性。结论　HLA DRB10 90 1、12等位基因均与湖北省汉族人胃腺癌呈正相关 ,而HLA DRB115则呈负相关。上述各相关的HLA DRB1等位基因与胃腺癌患者患病年龄、性别、TNM分期、肿瘤细胞分化程度无关 ;Hp感染虽为胃癌重要的致病因素 ,但并不是HLA DRB1等位基因通过增加Hp感染危险性 ,而影响胃腺癌的遗传易感性。Objective To investigate the association of HLA DRB1 alleles with gastric carcinoma and H. pylori infection. Methods HLA DRB1 alleles polymorphism in 63 patients with gastric adenocarcinoma and 136 normal controls were tested with the polymerase chain reaction/sequence specific primer(PCR/SSP) technique. The status of H. pylori infection was determined by histology of gastric biopsy specimens and /or by ELISA. Results Both HLA DRB1 0901 and HLA DRB1 12 were positively associated, and HLA DRB1 15 was negatively associated with gastric adenocarcinoma, but no significant associations were found between patients with and without above HLA DRB1 alleles with regard to patients' age at presentation, sex ratio, tumor site (distal vs proximal), TNM staging, histological grading of tumor or status of H. pylori infection. Conclusions Our results suggest that HLA DRB1 0901, DRB1 12 and DRB1 15 are associated with gastric adenocarcinoma, but are not associated with the clinical features of gastric adenocarcinoma and the status of H. pylori infection.

关 键 词：人类白细胞抗原 序列分析 DNA 胃癌 幽门螺杆菌 遗传易感性 

分 类 号：R735.2[医药卫生—肿瘤]