转基因烟草中的海藻糖测定  

DETECTION OF TREHALOSE IN TRANSGENIC TOBACCO BY HPLC WITH ELSD

作　　者：周坚[1] 杨波[1] 戴秀玉[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2001年第3期378-380,共3页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目 ( 3 9980 0 2 3 )&&

摘　　要：The \%E.coli\% trehase synthalose gene(\%otsA\%) was transferred into \%Nicotiana tabacum\% mediated by \%Agrobacterium\%, but the method for detecting low concentration of trehalose in transgenic plant was not available.The high performance liquid chromatograph(HPLC) with evaporative light\|scatting detector (ELSD) using water:methyl cyanide(1∶2\^6 v/v) as mobile phase was established in this work. An ODS column Zorbax RX\|SIL was employed. the trehalose detection limits of ELSD was 5mg/L. From the linear relationship between the logarithm of trehalose concentration and the logarithm of peak area, it was shown there was 14\^7μg\5(g FW)\+\{-1\} in transgenic plant. The data strongly confirmed that trehalose was responsible for the improved stress tolerance of the tobacco.The \%E.coli\% trehase synthalose gene(\%otsA\%) was transferred into \%Nicotiana tabacum\% mediated by \%Agrobacterium\%, but the method for detecting low concentration of trehalose in transgenic plant was not available.The high performance liquid chromatograph(HPLC) with evaporative light\|scatting detector (ELSD) using water:methyl cyanide(1∶2\^6 v/v) as mobile phase was established in this work. An ODS column Zorbax RX\|SIL was employed. the trehalose detection limits of ELSD was 5mg/L. From the linear relationship between the logarithm of trehalose concentration and the logarithm of peak area, it was shown there was 14\^7μg\5(g FW)\+\{-1\} in transgenic plant. The data strongly confirmed that trehalose was responsible for the improved stress tolerance of the tobacco.

关 键 词：高效液相层析 蒸发光散射检测器 转基因烟草 海藻糖 检测 

分 类 号：Q533[生物学—生物化学]