重金属污染土壤毒性的发光菌法诊断  

Photobacterium phosphoreum assay on the toxicity of soil contaminated by heavy metals.

作　　者：李彬[1] 李培军[1] 王晶[1] 杨桂芬[1] 张海荣[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,沈阳110015

出　　处：《应用生态学报》2001年第3期443-446,共4页Chinese Journal of Applied Ecology

基　　金：国家重点基础研究发展规划项目! (G1 9990 1 1 80 8 5);中国科学院重大资助项目! (KZ951 B1 2 0 7 0 1 0 3)

摘　　要：应用明亮发光杆菌T3(PhotobacteriumPhosphoreum )对重金属污染土壤的毒性进行诊断 ,确定了实验室中采用人为定量投加污染物的情况下 ,土壤的最佳平衡时间为 2 4h ,最佳浸提时间为 2h ,最佳浸提剂为 0 .1mol·L-1Hcl.对单一和复合污染条件下土壤毒性研究表明 ,单一重金属污染条件下 ,重金属的投加量与发光菌的发光度间存在明显的相关性 ,根据剂量效应曲线得出 4种重金属的EC50 分别为Cd 2 6 .12mg·kg-1、Cu 2 91.48mg·kg-1、Zn 72 .46mg·kg-1和Pb 2 174.93mg·kg-1,复合污染条件下 ,由于金属的协同作用 ,毒性明显增加 ,从而为污染土壤的优先修复提供了理论依据 .The toxicity of soil artificially contaminated by heavy metals was assessed with Photobacterium phosphoreum.The results showed that the duration of optimum balance was 24 hours, and that of optimum extraction was 2 hours. Two leaching methods were compared, and data showed that optimum leaching solvent was hydrocarbon chloride with a concentration of 0.1mol·L -1 HCL. Dose response curve indicated that the content of heavy metals was positively related with the toxicity of soil. The general detection limit given by EC 50 of soil contaminated by single heavy metal were Cd 26.12 mg·kg -1 , Cu 291.48 mg·kg -1 ,Zn 72.46 mg·kg -1 and Pb 2174.93 mg·kg -1 . This study also indicated the toxicity of complex heavy metals pollution in soil was stronger than that of individual element. The research could provide a theoretic base for the remediation of soil contaminated by heavy metals.

关 键 词：毒性 发光菌法 土壤污染 重金属污染 诊断 

分 类 号：X171.5[环境科学与工程—环境科学]