捕获肿瘤抗原的树突状细胞治疗小鼠膀胱肿瘤  

Dendritic cells activated by tumor antigen to treat bladder tumor in mouse

作　　者：陈仁富[1] 谢叔良[1] 

机构地区：[1]徐州医学院附属医院泌尿外科,江苏徐州221002

出　　处：《中国癌症杂志》2001年第3期264-264,共1页China Oncology

摘　　要：目的 :研究捕获肿瘤抗原的树突状细胞 (dendriticcells,DC)治疗膀胱肿瘤的可行性。方法 :分离小鼠脾脏树突状细胞 ,体外经粒 巨细胞集落刺激因子 (GM CSF)活化和放射线灭活的肿瘤细胞刺激后 (D组 ) ,治疗膀胱肿瘤移植模型 ,并设立空白对照组 (A) ,单纯GM CSF活化的树突状细胞组 (B)及灭活肿瘤细胞组 (C)。结果 :D组特异性T淋巴细胞活性、IL 4及IFN γ水平明显高于其它组 (P <0 0 1) ;所有小鼠长期无瘤存活 (>10 0天 ) ,而其它组小鼠存活期小于 30天 (P <0 0 0 1)。结论Purpose:To study the feasibility of GM CSF and the tumor antigen activated dendritic cells in treating bladder tumor in rats.Methods:The DCs isolated from the spleen of T739 mice which were stimulated in vitro by syngenic granulocyte macrophage colony stimulating factor (GM CSF) and irradiated inactivated BTT739 Bladder tumor cells(Group D),were transfused intravenously once a week when BTT739 tumor cells were inoculated in mouse. Group A was established as control,group B and C were injected with GM CSF activated DCs and inactivated tumor vaccine.Results:In group D, the CTL activity against the BTT739 tumor and cytokine IL 4 and IFN γ was significantly higher than those in other groups ( P <0 01). The growing BTT739 tumors disappeared dramatically and the murine survival intervals were significantly longer than those in other groups.Conclusions:The results suggest that the inoculation of GM CSF and tumor antigen activated DCs could be an effective new method for the treatment of bladder tumors and possesses a favorable prospect for clinical application. [

关 键 词：树突状细胞 膀胱肿瘤 粒-巨细胞集落刺激因子 免疫治疗 

分 类 号：R737.14[医药卫生—肿瘤] R73－362[医药卫生—临床医学]