榄香烯复合瘤苗对Hca-F肝癌小鼠的免疫治疗效应及对IL-10与IL-12分泌的影响  

Immunotherapeutic Effects of Elemene Combo Tumor Cell Vaccine on Hca-F and Changes of IL-10 and IL-12 Secretion by Splenocytes of Treated Mice

作　　者：赵卫红[1] 施广霞[1] 袁小林 钱振超[1] 

机构地区：[1]大连医科大学肿瘤生物治疗研究所,辽宁大连110627 [2]大连第三人民医院肿瘤内科

出　　处：《中国肿瘤生物治疗杂志》2001年第2期126-126,共1页Chinese Journal of Cancer Biotherapy

基　　金：国家自然科学基金重点项目 ( 39730 44 0 II)

摘　　要：目的 :探讨榄香烯复合瘤苗 (EC TCV)对小鼠肝癌Hca F的免疫治疗效应及治疗小鼠脾细胞IL 10与IL 12分泌的变化。方法 :带Hca F小鼠分别用EC TCV ,CY ,EC TCV +CY治疗或不治疗 (PBS对照 ) ,观察比较肿瘤生长情况并用ELISA法测脾细胞与Hca F细胞混合培养上清中的IL 10与IL 12。结果 :EC TCV免疫治疗或CY化疗后 ,肿瘤潜伏期延长 ,瘤重减轻 ,用EC TCV +CY进行化免治疗的小鼠在第 15天活杀时均无瘤生长 ,与Hca F细胞混合培养的EC TCV治疗小鼠脾细胞培养上清中IL 10浓度明显降低 ( 1.82± 0 .2 9pg/ml,P <0 .0 5 ) ,IL 12浓度明显升高 ( 7.13± 1.89pg/ml,P <0 .0 5 )。结论 :EC TCV对Hca F有免疫治疗效应 ,可与CY并用而加强 ,而EC TCV主动免疫促进脾细胞IL 12分泌、抑制IL 10分泌可能是其抗瘤作用的机制之一。Objective: To investigate the immunotherapeutic effects of elemene combo tumor cell vaccine(EC TCV) on Hca F murine hepatic carcinoma and changes of IL 10 and IL 12 secretion by splenocytes of treated mice. Methods: BALB/c mice inoculated with Hca F were treated with EC TCV, CY, EC TCV+CY or PBS control, tumor growth was measured, concentration of IL 10 and IL 12 in supernatants of mixed splenocytes and Hca F culture(MSTC) was assayed with ELISA. Results: After EC TCV immunotherapy and CY chemotherapy, the latent period of tumor growth prolonged, mean tumor weight (MTW) decreased, even no nodule was observed in EC TCV+CY chemoimmunotherapy group, the concentration of IL 10 in supernatants of MSTC of EC TCV immunotherapy group decreased significantly(1.82±0.29 pg/ml, P <0.05), and that of IL 12 increased significantly (7.13±1.89 pg/ml, P <0.05).Conclusion: Immunization with EC TCV exhibited immunotherapeutic effect on Hca F which could be enhanced by combination therapy with CY and stimulating IL 12 secretion and suppressing IL 10 secretion of EC TCV immunized murine splenocytes might be one of its anti tumor mechanisms.

关 键 词：榄香烯 瘤苗 免疫治疗 IL-10 IL-12 小鼠Hca-F 肝癌 

分 类 号：R735.7[医药卫生—肿瘤] R730.51[医药卫生—临床医学]