两种干扰素抗Tca81 1 3、ACC- M体外增殖的比较研究  

Comparison of Anti Proliferation Effects of Two IFNs on Tca8113, ACC-M in Vitro

作　　者：林李嵩[1] 林国础[1] 陈万涛[1] 周晓健[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔颌面外科,上海200011

出　　处：《上海口腔医学》2001年第2期112-115,共4页Shanghai Journal of Stomatology

基　　金：国家自然科学基金 (3 9670 787)

摘　　要：目的　比较干扰素α和干扰素γ分别对舌鳞状细胞癌 (Tca8113)和腺样囊性癌 (ACC M)直接抗癌作用及抗癌特异性。方法　采用MTT法和集落法 (HTCA) ,分别检测两种干扰素对Tca8113、ACC M及L92 9细胞增殖活性的影响。结果　 10 0 0U/ml干扰素α、干扰素γ对Tca8113细胞生长抑制率分别为 (4 5 .4± 4.1) % ,(4 5 .9± 5 .4) % ;集落形成抑制率分别为 (5 2 .0± 2 .9) % ,(5 4.6± 4.1) % ;对ACC M细胞生长抑制率分别为 (2 3.1± 2 .1) % ,(2 6 .5±1.3) % ;集落形成抑制率分别为 (2 6 .2± 2 .0 ) % ,(2 7.3± 1.8) %。两种干扰素之间无显著性差异 (P >0 .0 5 ) ,两种细胞系 (株 )之间有显著性差异 (P <0 .0 5 )。 10 0 0U/ml干扰素α、干扰素γ对L92 9细胞生长抑制率分别为 (3.9± 1.1) % ,(4 .1± 0 .7) % ,与Tca8113相比有显著性差异 (P <0 .0 1)。两种干扰素均有较好的抗癌作用特异性。结论　干扰素α、干扰素γ对舌鳞状细胞癌有较强的直接抗癌作用和较好的抗癌特异性 。Objective Comparison of anti proliferation and anti tumor specificity of two IFNs on Tca8113, ACC M in Vitro. Methods MTT assay and HTCA were performed to determine direct anti proliferation effects of two IFNs on Tca8113, ACC M and L929 cells, respectively.Results When Tca8113 cells were treated with 1000U/ml α IFN, γ IFN, respectively, cell proliferation inhabition rate was (45.4±4.1)% by γ IFN, (45.9±5.4)% by γ IFN; inhibition rate of colony formation was (52.0±2.9)% by α IFN, (54.6±4.1)% by γ IFN; and ACC M cell proliferation inhibition rate was (23.1±2.1)% by α IFN, (26.5 ±1.3)% by γ IFN; inhibition rate of colony formation was (23.1±2.1)% by α IFN, (26.5 ±1.3)% by γ IFN; inhibition rate of colony formation was (26.2±2.0)% by α IFN, (27.3±1.8)% by γ IFN. There was no significant difference between two IFNs ( P >0.05). When L929 cells were treated with 1000U/ml α IFN, γ IFN, respectively, cell proliferation inhibition rate was (3.9±1.1)% by α IFN, (4.1±0.7)% by γ IFN; There was significant difference in antiproliferation on Tca8113 cells and L929 cells ( P <0.01). Conclusions Both α IFN and γ IFN have better direct anti cancer effect on tongue squamous cell cancer and anti tumor specificity. But anti proliferation effect of two IFNs on ACC M cells was not obvious. [

关 键 词：干扰素 口腔鳞癌 腺样囊性癌 增殖 

分 类 号：R739.8[医药卫生—肿瘤]