乙醇对清醒兔Oddi括约肌运动功能的影响  

Effect of ethanol on sphincter of Oddi motility in conscious rabbits

作　　者：杨春敏[1] 毛高平[1] 朱明[1] 张秀荣[1] 张映辉[1] 

机构地区：[1]中国人民解放军空军总医院消化科,北京市100036

出　　处：《世界华人消化杂志》2001年第6期653-657,共5页World Chinese Journal of Digestology

摘　　要：目的酒精是引起胰腺炎的常见原因。本文观察乙醇是否影响清醒兔Oddi括约肌的运动。方法健康白兔8只,在无菌条件下理置Oddi括约肌测压管,手术后恢复7d进行实验,将测压管连接于低顺应性毛细管水灌注系统,记录Oddi括约肌压力变化。经十二指肠分别灌流50mL·L^1,150mL·L^1和300mL·L^1乙醇30min,灌流速度0.5mL·min^1;静脉则注射同样浓度的乙醇,以生理盐水作为对照,计算灌流乙醇前后Oddi括约肌运动参数变化。结果①经十二指肠灌流150mL·L^1和300mL·L^1的乙醇后,Oddi氏括约肌基础压分别为(1.71+0.33)kPa和(1.13±0.17)kPa。明显低于生理盐水对照组(2.32+0.57)kPa(P<0.05和P<0.01),并且呈浓度依赖关系.十二指肠灌流150mL·L^1和300mL·L^1的乙醇后Oddi括约肌位相收缩的振幅分别为(5.64±1.94)kPa和(3.68±1.51)kPa频率分别为(2.8±1.1)次·min^1和(1.9±0.7)次min^1、动力指数分别为(4.44±1.69)kPa·s和(3.56±1.54)kPa·s与生理盐水对照振幅、频率和动力指数分别为(7.68±2.45)kPa、(1.9+0.7)次·min^1和(6.07+1.69)kPa·s_1比较也明显降低(P<0.05和P<(0.01)、经静脉灌流150mL·L和300mL·L^1的乙醇后.Oddi括约肌基础压力明显低于生理盐水对照!(1.56±0.28)kPa和(1.09±0.21)kPa vs(2.23±0.46)kPa](P<0.05和P<0.01)静脉灌流150mL·L^1后Oddi括约肌位相收缩的振幅、频率和动力指数分别为(6.34+2.20)kPa,(2.7+1.0)次·min^1和(5.01±1.93)kPa·s.灌流300 mL·L^1乙醇后三个参数分别为(4.21±1.76)kPa、(2.1+0.8次· min^(-1)和(4.18±1.78)kPa·s,与生理盐水对照比较均明显降低(P<0.05和P<0.01)。③经十二指肠灌流150mL·L^(-1)和300mL·L^(-1)的乙醇后Oddi括约肌位相收缩的周期时间分别为(7.4±2.1)s和(8.3±2.5)s,经静脉灌流同样浓度的乙醇后分别为(7.2±2.3)s和(8.6±2.7)s,与生理盐水对照比较,均有明显增加(P<0.01)。④比较经十二指肠或经静脉两种途径灌流乙醇对Oddi括约肌运动的影响,发现两者之间无明显差�AIM Alcohol is one of the factors which induces pancreatitis. This study was to observe whether ethanol would affect sphincter of Oddi (SO) motility. METHODS A manometric catheter for measuring SO pressure and a silastic cannula for drugs were aseptically implanted into the common bile duct and the midduodenum respectively in each of 8 rabbits. Experiment began 7 days after recovery from operation. The changes of SO pressure was recorded in both ethanol group and control group, using low compliance capillary water perfusion system. Various doses of ethanol (50mL·L^1. 150mL·L^1. 300mL·L^1) each were infused intraduodenally (id) or intravenously (iv) within 30 min at a rate of 0.5 mL·min in two-three separate days. Normal saline was given instead of ethanol in control group. RESULTS ① Basal pressure of SO was 1.71±0.33 kPa in id 150mL·L^1 ethanol group, and 1.13±0.17 kPa in id 300mL·L^1 ethanol group, both decreased significantly as compared with that in control 2.32±1.57 kPa (P<0.05, P<0.01). Amplitudes, frequencies and motility indexes of phasic contraction in both groups were 5.64±1.94 kPa and 3.68±1.51 kPa, 2.8±1.1min and 1.9±0.7min, 6.07±1.69 kPa·s and 4.44±1.69 kPa·s. All these parameters were lower than those in saline control group (Amplitude, frequency and motility index were 7.68±2.45 kPa, 1.9±0.7 min and 3.56±1.54 kPa, respectively. P<0.05). ② Basal pressure of SO was (1.56±0.28) kPa in iv 150mL·L ethanol group, and 1.09±0.21 kPa in iv 300mL·L ethanol group, both decreased as compared to control (P<0.05, P<0.01), Amplitude, frequency and motility index of SO phasic contractions in iv 150mL·L ethanol group were 6.34±2.20kPa, 2.7±1.0) min and 5.01±1.93 kPa·s, and in iv 300mL·L ethanol group were 4.21±1.78kPa, 2.1±0.8min and 4.18±1.65 kPa· s, respectively. All above parameters were lower than those in control group (P<0.05, P<0.01). ③ Durations of contractions of SO in id 150mL·L or 300mL·L ethanol group were 7.4+2.1 s and 8.3+2.5 s, and in iv 150mL·L or 300mL·L e

关 键 词：奥狄氏括约肌运动 药物作用 乙醇 药理学 括约肌切开术 胰腺炎 病因学 治疗 

分 类 号：R575.6[医药卫生—消化系统]