用光声光谱技术测量鼻咽癌细胞的光谱吸收特性  

PHOTOACOUSTIC ABSORPTION CHARACTERISTICS OF SUNE-1, CNE-2,AND CNE-1 HUMAN NASOPHARYNGEAL CARCINOMA CELL LINESMEASURED USING PHOTOACOUSTIC TECHNIQUES

作　　者：唐志列[1] 夏云飞[2] 李满枝[2] 刘金龙[1] 裴红津[1] 

机构地区：[1]华南师范大学物理系,广东广州530631 [2]中山医科大学肿瘤防治中心,广东广州510060

出　　处：《生物物理学报》2001年第2期241-244,共4页Acta Biophysica Sinica

基　　金：广东省自然科学基金资助项目!(970088;980046)

摘　　要：为了解人鼻咽癌细胞的光谱吸收特性,用双光束归一化光声光谱技术测量三株人鼻咽癌细胞 (SUNE -1 ,CNE -2 ,CNE -1)的归一化吸收光谱和光谱吸收系数。结果表明 :(1)在波长4000～6000 范围内 ,这三株人鼻咽癌细胞的光谱吸收系数大小为 :SUNE -1>CNE -2>CNE -1 ;(2)在波长4200 处平均相对光声信号值为 :SUNE -1 :1.36 ,CNE -2 :1.20,CNE -1 :1.10 ,三者之间的差异具有显著性 (P值=0.012)。人鼻咽癌细胞株SUNE -1 ,CNE -2和CNE -1具有不同的光声光谱特性。Purpose: To investigate optical absorption characteristics of human nasopharyngeal carcinoma cell lines. Methods: Standardized photoacoustic spectroscopy and optical absorption coefficient of SUNE-1,CNE-2,and CNE-1 were measured respectively using double beam standardized photoacoustic technique. Results:(1)in the range from 4000? to 6000?,optical absorption coefficient of three cell lines are in the following order: SUNE-1>CNE-2>CNE-1;(2)At wavelength 4200?, the mean values of relative photoacoustic signal for SUNE-1, CNE-2, and CNE-1 are 1.36, 1.20,and 1.10 respectively, showing a singnificant difference(p=0.012). Conclusion: Human nasopharyngeal carcinoma cell lines SUNE-1, CNE-2, and CNE-1 have different photoacoustic spectroscopic characteristics. This study lays a foundation of further research on biological behaviors of nasopharyngeal carcinoma.

关 键 词：光声光谱 光谱吸收系数 鼻咽癌细胞 生物学行为 

分 类 号：R73－33[医药卫生—肿瘤] Q279[医药卫生—临床医学]