不同脱乙酰度和分子量壳聚糖的神经亲和性研究  

STUDIES ON NERVE AFFINITY OF CHITOSAN WITH DIFFERENTDEACETYLATION DEGREE AND MOLECULAR WEIGHT

作　　者：李训虎[1] 王红武[1] 龚海鹏[1] 公衍道[1] 赵南明[1] 张秀芳[1] 

机构地区：[1]清华大学生物科学与技术系

出　　处：《生物物理学报》2001年第2期253-260,共8页Acta Biophysica Sinica

基　　金：国家863计划基金资助!(编号 :819 -07-01)

摘　　要：研究了四种不同脱乙酰度和分子量的壳聚糖与神经细胞的亲和性。利用酶联免疫技术(ELISA)测定了人血清白蛋白和纤粘连蛋白在四种壳聚糖膜上的吸附量 ,并利用圆二色性 (CD)测定了人血清白蛋白吸附在四种壳聚糖膜上的二级结构。通过研究它们对胎鼠大脑皮层细胞(FMCC)生长的影响 ,发现在这四种壳聚糖中 ,分子量较大的两种壳聚糖更有利于神经细胞的生长 ,许多轴突聚集成树干状。In this paper four types of chitosan with different molecular weight and deacetylation degree were used, and their effects on the growth of fetal mouse cerebral cortex (FMCC) cells were studied. Adsorption amounts of albumin and fibronectin on chitosan membranes were measured by means of Enzyme Linked Immunosorbent Assay (ELISA), and the circular dichroism (CD) spectra of albumin adsorbed on chitosan membranes were also measured. Results indicated that in these four types of chitosan, the higher degree of deacetylation, the more protein adsorbed on membranes. The secondary structure of albumin adsorbed on the first type of chitosan was significantly different from that on the other three types of chitosan.Axons growed faster on the two types of chitosan with higher molecular weight.

关 键 词：壳聚糖 神经亲和性 分子量 脱乙酰度 神经细胞 

分 类 号：Q421[生物学—神经生物学] R318.08[生物学—生理学]