球孢白僵菌高壳聚糖酶突变株的筛选  

SCREENING OF HIGHER CHITOSANASE-PRODUCING MUTANT OF BEAUVERIA BASSIANA

作　　者：方祥年[1] 杜昱光[2] 黄秀梨[3] 洪泂[3] 

机构地区：[1]浙江大学生命科学院,杭州310027 [2]中国科学院大连化学物理研究所,大连116023 [3]北京师范大学生物系,北京100875

出　　处：《微生物学通报》2001年第3期60-64,共5页Microbiology China

基　　金：国家九五攻关项目! (No . 96 C0 3 0 1 0 1 1 )

摘　　要：以球孢白僵菌 (Beauveriabassiana) 1 31 6为出发菌株 ,通过紫外线和高能电子束分别诱变分生孢子 ,采用平板透明圈法初筛和摇瓶培养复筛的方法 ,经 3轮诱变 ,筛选到 5株高产突变株。其中一株最高产的定名为Beauveriabassiana 1 31 6 V1 ,其壳聚糖酶活力是原始菌株的 1 6倍 ;而且经传代培养 。The conidia of Beauveria bassiana 1316 were mutagenized by UV and energy\|rich electron beam injection.Through three times mutagenesis five higher chitosanase\|producing mutant strains were obtained by screening with the method of transparent zones and chitosanase activity measurement of culture filtrate.The highest\|yield mutants chitosanase activity was raised 16 times compared with the original strain.It was named as Beauveria bassiana 1316\|V1 Its higher chitosanase\|producing ability could be inherited after several subcultures.

关 键 词：球孢白僵菌 壳聚糖酶 诱变 突变株 筛选 

分 类 号：Q939.9[生物学—微生物学]