番鸭细小病毒强、弱毒株VP2基因的序列测定比较  

Sequencing of DNA Encoding VP2 Structural Protein of Wild-type and Attenuated Strains of Muscovy Duck Parvovirus

作　　者：娄华[1] 白挨泉[1] 顾万军[1] 贺东生[2] 杨德威[2] 刘福安[2] 

机构地区：[1]佛山科技学院动物医学系,广东南海大沥528231 [2]华南农业大学动物医学系,广东广州五山510642

出　　处：《病毒学报》2001年第2期175-179,共5页Chinese Journal of Virology

基　　金：广东省高等教育厅( 9742 )

摘　　要：According to the complete nucleotide sequence of Muscovy duck parvovirus registered in the gene bank, two modified primers (LHMP7/LHMP8) were designed and, for each of them, a restriction endonuclease recognition site, SacⅡ or KpnⅠ, was included respectively. The DNA encoding VP2 structural protein of the wild strain MDPV Q and the attenuated strain MDPV 26 which had been derived by continued passage of virulent wild type (MDPV Q) in Muscovy duck embryo were amplified by PCR and recombined into T vector and sequenced respectively. It shows that both DNA encoding VP 2 structural protein of MDPV Q and MDPV which has been registered in the gene bank had the high homology (97.9%). MDPV 26 and MDPV Q displayed 99.7% identity, and shared 98.3% homology with that of reported MDPV.According to the complete nucleotide sequence of Muscovy duck parvovirus registered in the gene bank, two modified primers (LHMP7/LHMP8) were designed and, for each of them, a restriction endonuclease recognition site, SacⅡ or KpnⅠ, was included respectively. The DNA encoding VP2 structural protein of the wild strain MDPV Q and the attenuated strain MDPV 26 which had been derived by continued passage of virulent wild type (MDPV Q) in Muscovy duck embryo were amplified by PCR and recombined into T vector and sequenced respectively. It shows that both DNA encoding VP 2 structural protein of MDPV Q and MDPV which has been registered in the gene bank had the high homology (97.9%). MDPV 26 and MDPV Q displayed 99.7% identity, and shared 98.3% homology with that of reported MDPV.

关 键 词：番鸭细小病毒 VP2基因克隆 序列测定 

分 类 号：S858.325.3[农业科学—临床兽医学] S852.659.2[农业科学—兽医学]