巢式RT—PCR检测乳腺癌前哨淋巴结微转移的研究  

Detection of Breast Cancer Micrometastases of Sentinel Nodes by Means of Nested-RT-PCR

作　　者：周新惠[1] 黄钟英[1] 邬一军[1] 

机构地区：[1]浙江大学医学院附属第一医院普外科,杭州市310003

出　　处：《中国肿瘤临床》2001年第5期358-360,共3页Chinese Journal of Clinical Oncology

摘　　要：目的：探讨乳腺癌前哨淋巴结（ＳＬＮ）定位和腋淋巴结微转移检测的临床意义。方法：对２０例乳腺癌患者术中肿块周围注射美蓝定位前哨淋巴结，用巢式ＲＴ－ＰＣＲ法检测腋淋巴结中Ｍａｍｍａｇｌｏｂｉｎ ｍＲＮＡ的表达。结果：ＳＬＮ定位成功率为８５．０％（１７／２０），ＳＬＮ与非ＳＬＮ组微转移的检出率具显著差异（Ｐ＜０．０１）。在常规病检阴性的淋巴结中，巢式 ＲＴ—ＰＣＲ法微转移的检出率为 １５．６％（ １７／１０９）。结论：巢式ＲＴ—ＰＣＲ法较常规病理检查更为敏感，通过ＳＬＮ定位和巢式ＲＴ—ＰＣＲ的联合使用，可明显提高乳腺癌腋淋巴结微转移的检出效率。Objective: To investigate the clinical significance of locating sentinel lymph nodes (SLN) and detecting the axillary lymph node micrometastases in breast cancer. Methods: In 20 breast cancer patients, methylene blue was injected into the breast parenchyma to locate the SLNs, and nested-RT-PCR was used to examine the expression of mammaglobin mRNA. Results: The SLN was successfully identified in 17 of 20 cases (85%), the micrometastases detection between groups of SLNs and non-SLNs had significant difference (P<0.01). In the routine pathological examination of negative lymph nodes, the detection rate of micrometastases was 15.6% (17/109) by nested-RT-PCR. Conclusion: The mammaglobin nested-RT-PCR is more sensitive than routine pathological method for the detection of micrometastases. The combined utilization of locating SLN and nested-RT-PCR can greatly enhance the efficiency of micrometastases detection in axillary lymph nodes.

关 键 词：乳腺肿瘤 前哨淋巴结微转移 聚合酶链反应 RT-PCR 

分 类 号：R737.9[医药卫生—肿瘤] R73－37[医药卫生—临床医学]