MTT法评价镓合金的细胞毒性  

Evaluation on the cytotoxicity of Gallium alloy by MTT-assay

作　　者：徐钢梅[1] 张彩霞[2] 宁丽[2] 孙皎[2] 孟爱英[2] 

机构地区：[1]浙江大学医学院附属口腔医院正畸科,杭州310006 [2]上海生物材料研究测试中心

出　　处：《中华口腔医学杂志》2001年第3期189-192,共4页Chinese Journal of Stomatology

摘　　要：目的　研究新型不含汞的牙体充填材料镓合金的细胞毒性。方法　采用一种能快速评定细胞增殖率和细胞毒性的比色分析方法———MTT比色法 ,检测镓合金和高铜银汞合金对小鼠成纤维细胞L92 9细胞相对增殖率的影响 ,评价其细胞毒性。结果　参照上海市医用生物材料生物性能测试标准 ,镓合金表现出较高的细胞相对增殖率 ,2d时为 93 0 %± 4 9% ,4d时为 10 2 0 %± 3 5 % ,7d时为 10 7 2 %± 4 2 % ,其细胞毒性为 0级 ,即无明显细胞毒性 ;高铜银汞合金表现为中度细胞毒性。结论　镓合金无明显的细胞毒性。Objective To test the cytotoxicity of Gallium alloy, a new mercury free dental restorative material. Methods L 929 mouse fibroblasts were used to detect the cell relative proliferation rate of Gallium alloy and high copper amalgam by MTT assay. Results The results indecated that the relative growth rate induced by Gallium alloy was high (93 0%±4 9% 2ds, 102 0%±3 5% 4ds, 107 2%±4 2% 7ds), and the cytotoxicity of Gallium alloy was 0 grade according to the Test Standard of Shanghai Medical Biomaterial, meaning Gallium alloy had no significant cytotoxicity and the relative growth rate of Gallium alloy was higher than that of high copper amalgam.The high copper amalgam showed medium cytotoxicity. Conclusion Gallium alloy has no significant cytotoxicity.

关 键 词：镓合金 牙科合金 细胞毒性试验 MTT比色法 细胞增殖率 

分 类 号：R783.1[医药卫生—口腔医学]