川芎嗪注射液抗脑缺血-再灌流损伤作用机制的实验研究  

Experimental study on ligustrazini in the treatment of cerebral ischemiareperfusion injury

作　　者：王万铁[1] 陈寿权[2] 徐正衸[1] 林丽娜[3] 王卫[1] 李惠萍[2] 李东[4] 王宗敏[5] 

机构地区：[1]浙江温州医学院病理生理教研室,325027 [2]温州医学院附属一院急诊科 [3]温州医学院附属一院麻醉科 [4]温州医学院附属一院检验系分析中心 [5]温州医学院附属二院病理科

出　　处：《中华急诊医学杂志》2001年第3期182-184,189,共4页Chinese Journal of Emergency Medicine

基　　金：浙江省跨世纪学术和技术带头人培养基金项目(992 0 86 );浙江省中医科研计划重点课题基金项目(980 37)

摘　　要：目的　探讨川芎嗪注射液 (LGTI)对脑缺血 再灌流损伤 (CIRI)的防治作用及其机制。方法　制备家兔CIRI模型 ,随机分为假手术对照组、缺血 再灌流组和LGTI组 ,动态观察血浆及脑组织一氧化氮 (NO)水平、内皮素 (ET)含量、丙二醛 (MDA)浓度、超氧化物歧化酶 (SOD)活性及脑超微结构的变化。结果　脑缺血 再灌流期间 ,血浆和脑组织NO水平及SOD活性与缺血前比较明显下降 (P <0 0 5和 P <0 0 1) ,ET及MDA含量与缺血前比较显著升高 (P <0 0 5和 P <0 0 1) ,超微结构发生异常改变 ;使用LGTI后 ,上述各指标的异常变化明显减轻 ,与缺血 再灌注组相比差异有显著意义 (P <0 0 1)。结论　LGTI对CIRI具有良好的防护作用 ,其机制与提高机体NO水平。Objective To investigate the cerehral protective effect of ligustrazini injection(LGTI) and its mechanism during cerebral ischemia reperfusion injury(CIRI).Methods Using a CIRI model in 20 rabbits which were randomly divided into control group and LGTI group.Changes of several parameters were observed in plasma and brain during CIRI,including nitric oxide(NO),endothelin(ET),malondialdehyde(MDA),superoxide dismutase(SOD)and ultrastructure of the brain.Results NO level and SOD activity in plasma and cerebral tissue all decreased significantly(P<005 and P<001),ET level and MDA content in plasma and cerebral tissue increased markably in period cerebral ischemia reperfusion(P<0 05 and P<001).There were obvious abnormal changes of brain ultrastructure during CIRI.When LGTI was administered at 20 minutes before ischemia and at begining of reperfusion,the changes of the above indices were ameliorated markedly than that of CIRI(P<0 01).Conclusion LGTI could effectively protect the brain form ischemia reperfusion injury,the mechanism was related to its raising NO level and reducing ET or oxygen free radicals.

关 键 词：川芎嗪注射液 脑缺血 再灌注损伤 一氧化氮 内皮素 氧自由基 

分 类 号：R741.05[医药卫生—神经病学与精神病学]