大肠杆菌海藻糖合成酶基因对提高烟草抗逆性能的研究  

EXPRESSION OF otsA GENE IN TOBACCO AND IMPROVEMENT SRESS TOLERANCE

作　　者：戴秀玉[1] 王忆琴[1] 杨波[1] 周坚[1] 

机构地区：[1]中国科学院微生物研究所,北京100080

出　　处：《微生物学报》2001年第4期427-431,共5页Acta Microbiologica Sinica

基　　金：国家自然科学基金资助项目! (3 9980 0 2 3 )&&

摘　　要：编码大肠杆菌海藻糖合成酶的otsA基因由农杆菌介导引入野生型烟草植株并在花椰菜花叶病毒启动子序列 (CaMV35S)控制下获得表达。蒸发光散射高效液相层析法测定海藻糖实验表明 ,转基因烟草能够合成海藻糖 ,合成量达 1 4μg g叶片湿重 ;转基因烟草表现为耐盐性生长、干燥失重缓慢等抗逆表型。说明海藻糖合成酶otsA基因的引入 ,改变了烟草的糖代谢途径 ,同时也提高了植物的耐盐碱、耐干旱特性。The Escherichia coli trehalose\|6\|phosphate synthase gene( otsA )was engineered under the control of cauliflower mosaic virus regulatory sequences (CaMV35S) for expression in plants. OtsA gene was incorporated into the chromosome DNA by Agrobacterium \|mediated transfer and expressed in Nicotiana tabacum .The otsA gene transgenic plant exhibited multiple phenotypic alteration:improved stunted growth and drought tolerance.The detached leaves from the transgenic plant,their water loss slower than that of the controls.Trehalose accumulated in transgenic plant was determined by HPLC suggest that synthesis of the sugar improving plant to stress tolerance.

关 键 词：otsA基因 海藻糖 转基因烟草 抗逆性能 

分 类 号：Q789[生物学—分子生物学]