Epstein-Barr病毒bhrfl表达对鼻咽癌细胞株CNE2拓扑异构酶Ⅰ表达的影响  

The effect of EB virus bhrfl gene expressing on the topoisomerase Ⅰ expression in nasopharyngeal carci noma cell line

作　　者：黄海[1] 潘星华 周江红[3] 温武[1] 周水淼[1] 余龙[4] 孔宪涛[5] 

机构地区：[1]第二军医大学附属长海医院耳鼻咽喉科,上海200433 [2]Yala University [3]第二军医大学附属长海医院药局,上海200433 [4]复旦大学遗传学研究所基因工程国家重点实验室 [5]第二军医大学附属长征医院全军临床免疫中心

出　　处：《中华实验和临床病毒学杂志》2001年第2期128-131,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：全军医药卫生基金资助项目 (96Q0 6 0 )

摘　　要：目的　了解Epstein Barr(EB)病毒基因bhrfl表达对鼻咽癌细胞拓扑异构酶Ⅰ表达的影响。方法　通过构建bhrfl表达载体并转染鼻咽癌细胞株CNE2细胞 ,以检测癌细胞在转染后其拓扑异构酶Ⅰ表达及其抗喜树碱诱发凋亡能力的改变。结果　bhrfl表达可增强癌细胞拓扑异构酶Ⅰ的表达 ,使癌细胞对喜树碱的敏感性下降 ,生存时间延长 ,凋亡率减少 (P <0 0 1)。结论　bhrfl的表达可通过增强CNE2细胞拓扑异构酶Ⅰ的表达而增强其抗喜树碱诱发的细胞凋亡作用。Objective To study the effect of bhrfl expressing on the topoisomerase Ⅰ expression in nasopharyngeal carcinoma cell line CNE2.Methods bhrfl recombinant expressing vector was constructed and used to transfect the nasopharyngeal carcinoma cell line CNE2.The CNE2 cells were then tested for changes of the topoisomerase Ⅰ expression and anti apoptotic ability under the treatment of camptothecin.Results bhrfl expressing could enhance the topoisomerase Ⅰ expression in CNE2 cells,that in end reduced cell sensitivity to camptothecin increased cell survival span and decreased apoptotic rate( P <0 01).Conclusion By increasing the expression of topoisomerase Ⅰ,bhrfl expressing can enhance the resistance of CNE2 cells to apoptosis induced by camptothecin.

关 键 词：疱疹病毒4型 病毒基因 拓扑异构酶Ⅰ 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]