我国各血清型轮状病毒非结构蛋白NSP4基因特征的初步分析  

Preliminary analysis of genetic characteristics of the nonstructural protein NSP4 of rotavirus strains with different serotypes in China

作　　者：徐倏燊[1] 王健伟[1] 张忠浩[2] 何金生[1] 董京芳[1] 温乐英[1] 洪涛[1] 

机构地区：[1]中国预防医学科学院病毒学研究所形态室,北京100052 [2]北京友谊医院儿科

出　　处：《中华实验和临床病毒学杂志》2001年第2期132-134,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家B63计划生物技术领域(102-07-02-06)；国家自然科学基金资助(30000145)

摘　　要：目的　对我国血清学调查中发现的四型 (G型 )轮状病毒NSP4序列进行测定和分析 ,了解NSP4的基因类型和变异状况。方法　利用银染测序方法对G1、G2、G3、G9型毒株NSP4cDNA序列进行测定。结果　四个血清型毒株NSP4的核苷酸和氨基酸序列的同源性分别为 78 8% ,93 5 % ;83 0 % ,95 5 %。G1、G3、G9型标本多属Wa组 ;G2型标本多属KUN组。NSP4的变异主要位于VP4结合域内的 135 145位氨基酸。结论　Wa组和KUN组是我国主要的两种NSP4基因型。Objective To study the genotypes and genetic variations of nonstructural protein NSP4 of rotavirus strains with different serotypes in China.Methods NSP4 cDNA of the four serotypes G1,G2,G3 and G9 were cloned and sequenced by SILVER SEQUENCE TM DNA Sequencing System.Results The identity of the nucleotide and amino acid were 78 8%-93 5%,83 0%-95 5%,respectively. NSP4 from strains G1,G3,G9 all fell into Wa allele, while that of G9 fell into KUN allele. Some variations of the amino acids were observed localizing at the position of 135-145 in the VP4 binding domain. Conclusion The results indicated that the two alleles Wa and KUN are the major prevalent NSP4 genotypes in China.

关 键 词：轮状病毒 NSP4 基因型 中国 

分 类 号：R373[医药卫生—病原生物学]