基因重组鼠伤寒沙门氏菌防龋疫苗的研究Ⅱ.携带变形链球菌pac基因表达质粒的构建与初步鉴定  

Recombinant Salmonella typhimurium Anticaries Vaccine Ⅱ. Construction and Confirmation of Expressing Plasmid Carrying A Region of S.mutans pac Gene

作　　者：陈罕[1] 凌均棨[2] 杨国平[1] 涂家珍[1] 

机构地区：[1]中山医科大学口腔医学院,510060 [2]中山医科大学口腔医学院,510060

出　　处：《华西口腔医学杂志》2001年第3期139-141,共3页West China Journal of Stomatology

基　　金：广东省自然科学基金资助项目 (编号 970 10 5 )

摘　　要：目的 :构建携带变形链球菌表面蛋白pac基因A区片段的原核高效表达系统。方法 :对含变形链球菌表面蛋白pac基因的pPC41质粒进行PCR扩增 ,获得了pac基因A区片段 ;将扩增获得pac基因A区片段与表达质粒pET17 b定向克隆 ,重组质粒用BamHI,EcoRV双酶切后电泳鉴定。结果 :获得携带pac基因A区片段的重组质粒。结论Objective:The objective of this study is to construct prokaryotic expressing plasmids carrying A region of S. mutans pac gene.Methods:The A region of pac gene was amplified by using PCR from plasmid pPC41 containing S.mutans surface protein pac gene. The new expression plasmid was constructed by using A region of pac gene and expressing plasmids pET17_b and directed cloning technique. The recombinant plasmid was identified by agarose gel electrophoresis after it was cut by using BamHI and EcoRV.Results:The expressing plasmid carrying pac gene A region was acquired.Conclusion:The recombinant plasmid provides useful experimental materials for further research work.

关 键 词：变形链球菌 表面蛋白 减毒鼠伤寒沙门氏菌 龋齿 基因重组 质粒 

分 类 号：R780.1[医药卫生—口腔医学]