机械拉伸对人牙周膜成纤维细胞Ⅰ型胶原及蛋白合成的影响  

Effects of Mechanical Stretch on Collagen Type Ⅰ and Total Protein Synthesis of Human Periodontal Ligament Fibroblasts

作　　者：陈新民[1] 韩文利[1] 赵云凤[1] 

机构地区：[1]四川大学华西口腔医学院,610041

出　　处：《华西口腔医学杂志》2001年第3期155-157,共3页West China Journal of Stomatology

摘　　要：目的 :研究拉伸应变对人牙周膜成纤维细胞Ⅰ型胶原及蛋白合成的影响。方法 :应用酶联免疫吸附实验及考马斯亮蓝微板法对拉伸应变下的细胞合成代谢变化进行观察 ,应变范围为 1%～ 8%。结果 :在 0 .5 %～ 8%拉伸应变范围内 ,人牙周膜成纤维细胞Ⅰ型胶原合成无明显变化 ,但 1%～ 8%拉伸应变作用下细胞蛋白合成减少 ,其中2 %拉伸应变影响最明显。结论Objective:The aim of this study is to investigate the effects of mechanical stretch on typeⅠcollagen and total protein synthesis of human periodontal ligament fibroblasts (PDLFs). Methods:Enzyme link immunosorbent assay (ELISA) and Coomassie brilliant blue stain method were used to examine the anabolism of PDLFs growing on the flexible culture plates subjected to mechanical stretch. The five loading devices of PDLFs were designed and the stain varied from 1% to 8%. Results:Mechanical stretch had no effect on type Ⅰ collagen synthesis. However, in the range of 1% to 8% of stretch, the protein synthesis was reduced. The mechanical stretching of 2% strain affected the protein synthesis most obviously. Conclusion:Mechanical stretch can modify the composition of extracellular matrix of PDLFs.

关 键 词：拉伸应奕 人牙周膜 成纤维细胞 Ⅰ型胶原合成 蛋白合成 

分 类 号：R780.2[医药卫生—口腔医学]