肺癌细胞与胞外基质选择裱衬表面粘附力学的研究  

Investigation on the Mechanics of Adhesion to the Selective Extracellular Matrix Coated Surfaces of Lung Cancer Cells

作　　者：张婷[1] 屈谦[1] 薛亚梅[1] 吴泽志[2] 宋关斌[2] 蔡绍皙[2] 

机构地区：[1]重庆医科大学第二临床学院呼吸内科,重庆400010 [2]重庆大学生物工程研究院,重庆400044

出　　处：《生物医学工程学杂志》2001年第2期320-322,F003,共4页Journal of Biomedical Engineering

摘　　要：肿瘤细胞与细胞外基质的粘附特性与肿瘤的侵袭转移密切相关 ,作者力图揭示人肺癌细胞相应的生物力学和生物流变学特征。采用微管吸吮技术定量测定体外培养的低转移人肺腺癌 (PAa)细胞和高转移人肺巨细胞癌 (PG)细胞与细胞外基质重要组份胶原蛋白 和层粘连蛋白 (L N)的粘附力学特性。结果表明 :与胶原蛋白 裱衬面的粘附力 ,在 PAa、PG细胞均较裱衬前增加 ,但在较低胶原浓度 (1.0 0 μg/ ml,2 .0 0 μg/ ml)时 PAa细胞的粘附力增加幅度大于 PG细胞 ;与 L N和固定浓度 (2 .0 0 μg/ m l)胶原蛋白 的复合裱衬面的粘附力为 PAa细胞大于 PG细胞 ,在较低 L N浓度 ,PAa(0 .6 2 5 μg/ m l)、PG(0 .6 2 5 μg/ ml;1.2 5 μg/ m l)细胞粘附力相对降低 ,尤以 PG细胞中的降低程度和幅度为大。因此 ,癌细胞与细胞外基质的粘附和去粘附行为主要通过膜受体介导 。The adhesion properties of tumor cells with extracellular matrix(ECM) are closely associated with their invasion and metastasis. Our work reported here was intended reveal the relevant biomechanical and biorheological manifestations of human lung cancer. Using micropipette aspiration technique, we investigated quantitatively the adhesive mechanics properties of high metastatic human giant cell carcinoma (PG) cells as well as low metastatic adenocarcinoma(PAa) cells of lung based on cell culture in vitro. The results showed that the adhesion forces of PAa and PG cells to collagen IV were significantly higher than those to glass surfaces, but at the lower concentrations (1.00μg/ml and 2.00μg/ml) of collagen IV, the amplitude for the increase of adhesion forces of PG cells were less than the amplitude for that of PAa cells, and most of the adhesion force values of PAa cells to the coated surfaces of incorporation of laminin along with 2 μg/ml collagen IV were significantly greater than those of PG cells. At the lower concentrations (0.625μg/ml for PAa cells, and 0.625 μg/ml, 1.25 μg/ml for PG cells) of laminin tested, the adhesion force values of PAa and PG cells all decreased, but the amplitude and level for the decreased values of adhesion forces of PG cells were greater than those for the PAa cells. In conclusion, the adhesive and proteolytic behaviour of cancer cells to extracellular matrix might be mediated mainly by tumor cell membrane receptors such as integrin receptors and laminin receptors, it might affect the biological characteristics and the metastasis of the tumor cells. The results may benefit to explain some questions in biomechanical views about how the highly metastatic PG cells are prone to migration and invasion.

关 键 词：肺肿瘤 细胞外基质 粘附 肿瘤转移 微管吸吮技术 生物力学 

分 类 号：R734.2[医药卫生—肿瘤] R318.01[医药卫生—临床医学]