核苷磷酸化酶的产酶条件优化的研究  

Study on Optimal Conditions of NPase

作　　者：闫蓬勃[1] 邱蔚然[1] 丁庆豹[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《药物生物技术》2001年第2期83-85,共3页Pharmaceutical Biotechnology

摘　　要：以鸟苷和 5 氟尿嘧啶为底物 ,乙酰短杆菌菌体为酶源酶法合成 5 氟尿苷。采用酵母浸膏培养基(酵母浸膏 4.0 % ,葡萄糖 0 .4% ,NaCl 0 .3 % ,pH7.0 ) ,较高的溶氧量 ,同时添加 0 .6 %的豆油 ,培养 12h ,产酶量可提高 30 %。5-Fluorouride was enzymatically synthesized from guanosine and 5-fluorouracil by brevibacterium acetylicum The suitable culture medium contained 40g yeast extract, 4g glucose, 3g NaCl in 1 liter while pH was adjusted to 7 0 with NaOH After brevibacterium acetylicum was cultured for 12 h in the condition of the higher dissolve oxygen and 0 6% soybean oil supplied , the amount of enzym produced can be increased by 30%

关 键 词：乙酰短杆菌 核苷磷酸化酶 5-氟尿苷 产酶条件 

分 类 号：Q555.4[生物学—生物化学]