天然抗氧化剂Isoverbascoside诱导人胃癌MGC 803细胞分化作用研究  

Differentiation of Human Gastric Cancer MGC 803 Cell Induced by Natural Antioxidant Isoverbascoside

作　　者：陈瑞川[1] 苏金华[1] 蔡克瑕[1] 付永贵[2] 李力[2] 

机构地区：[1]厦门大学生命科学学院抗癌研究中心,福建厦门361005 [2]厦门大学生命科学学院生物学系,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2001年第4期936-942,共7页Journal of Xiamen University：Natural Science

基　　金：福建省自然科学基金资助项目 (C9710 8)

摘　　要：人胃癌 MGC80 3细胞经天然抗氧化剂 Isoverbascoside(1sov)处理后 ,细胞生长和增殖呈现Isov浓度和处理时间依赖性抑制 ,群体倍增时间也相应延长 ;2 0 μmol/ L Isov处理可使细胞形态和超微结构发生正常化变化 ,集落形成率受抑 ;胃癌分化标志酶碱性磷酸酶和乳酸脱氢酶活性均呈时间依赖性下降 ,周期表现为 G1期阻滞 .表明 Isov具有诱导 MGC 80 3细胞分化的作用 。While treated with natural antioxidant lsoverbascoside (Isov),the growth and proliferation of human gastric carcinoma MGC 803 cell were markedly inhibited and the average cell population double time was correspondingly prolonged, with dependence on concentration and treatment time of Isov. Compared with control group, the cell morphology changed toward normality and cloning efficiency on soft agar was a1so inhibited while treated with 20 μmol/L Isov. As marker enzymes of differentiation,the activities of alkaline phosphatase (AKP) and 1actate dehydrogenase (LDH) were respectively down regulated, depending on treatment time. Detected by flow cytometer, the cell cycle distribution showed G 1 arresting.These results suggested that differentiation of MGC 803 cell induced by natural antioxidant Isov and may associate to cell cycle arresting.

关 键 词：抗氧化剂 胃癌细胞 诱导分化 Isoverbascoside 

分 类 号：R735.2[医药卫生—肿瘤]