番木瓜畸型花叶病毒(PLDMV)调查鉴定  

Identification of Papaya Leaf-distortion Mosaic Virus in South China

作　　者：贺国安[1] 肖火根[1] 真冈哲夫 张曙光[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室,广州510642 [2]日本国际农林水产业研究中心冲绳支所

出　　处：《热带作物学报》2001年第2期38-42,共5页Chinese Journal of Tropical Crops

基　　金：深圳市农业局资助项目(C990068);广东省自然科学基金项目(980126)

摘　　要：对采自广州、景洪（云南）、海口（海南）的各１７，２４，１３个番木瓜样品进行ＤＡＳ－ＥＬＩＳＡ、ＲＴ－ＰＣＲ和生物测定等分析鉴定，发现来自广州和海口的样品中有２１个样品感染番木瓜环斑病毒（ＰＲＳＶ），而来自景洪的样品中未发现感染ＰＲＳＶ，在所有样品中都未发现感染有番木瓜畸型花叶病毒的。Leaves of Papaya (Carica Papaya L.) with distorted and mosaic symptoms were collected with 17, 24 and 13 samples from Guangzhou, Jinghong and Haikou of south China respectively. These samples were examined by double antibody sandwich enzyme - linked immunosorbent assay (DAS-EIJSA) and by RT-PCR. It was found that 21 samples from Guangzhou and Haikou were infected with papaya ringspot virus. No sample was found infected with papaya leaf distortion mosaic virus (PLDMV).

关 键 词：番木瓜 畸型花叶病毒 调查 鉴定 DAS-ELISA RT-PCR 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治] S432.41[农业科学—植物保护]