褶合光谱法测定β-半乳糖苷酶的活性  

Determination of β-Galactosidase Activity by Convolution Spectrum Method

作　　者：郑红[1] 吴玉田[1] 方慧生[1] 

机构地区：[1]第二军医大学药学院药物分析教研室,上海200433

出　　处：《分析化学》2001年第5期583-585,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金资助项目 (No .3 9770 90 4)

摘　　要：运用褶合光谱法建立了 β 半乳糖苷酶的最佳褶合光谱以及该酶在最佳测试条件下的线性方程 :Qj=1.90 6× 10 -2 +4 .946× 10 -3 C +1.90 9× 10 -4 C2 ,r =1.0 0 0 0 ;对已瞬时转染 β 半乳糖苷酶表达载体的COS 1细胞提取液样品中的 β 半乳糖苷酶的活性进行了测定 ,并与吸光度值分析法相对照。结果表明 :本法快速、简便、准确 。The optimum convolution spectra of β galactosidase and the linear equation of Q j =1.906×10 -2 +4 946×10 -3 C +1.909×10 -4 C 2, r =1.0000 under the best condition were established by the convolution spectrum method. Based on the chromogenic substrate o nitrophenyl β galactoside, a method for the determination of β galactosidase activity in the COS 1 extraction sample which had been transfected transiently expression vector of β galactosidase was determined by using the convolution curve transformation technology. The convolution spectrum method was compared with the absorption method and the result was satisfactory. The method was simple, convenient, fast and valuable to the biochemical analysis.

关 键 词：褶合光谱法 Β-半乳糖苷酶 活性测定 生化分析 水解酶 

分 类 号：O657.3[理学—分析化学]